The effect of spike mutations on SARS-CoV-2 neutralization
Summary: Multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines show protective efficacy, which is most likely mediated by neutralizing antibodies recognizing the viral entry protein, spike. Because new SARS-CoV-2 variants are emerging rapidly, as exemplified by the B.1.1.7,...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124721002047 |
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Article |
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DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Chloe Rees-Spear Luke Muir Sarah A. Griffith Judith Heaney Yoann Aldon Jonne L. Snitselaar Peter Thomas Carl Graham Jeffrey Seow Nayung Lee Annachiara Rosa Chloe Roustan Catherine F. Houlihan Rogier W. Sanders Ravindra K. Gupta Peter Cherepanov Hans J. Stauss Eleni Nastouli Katie J. Doores Marit J. van Gils Laura E. McCoy |
| spellingShingle |
Chloe Rees-Spear Luke Muir Sarah A. Griffith Judith Heaney Yoann Aldon Jonne L. Snitselaar Peter Thomas Carl Graham Jeffrey Seow Nayung Lee Annachiara Rosa Chloe Roustan Catherine F. Houlihan Rogier W. Sanders Ravindra K. Gupta Peter Cherepanov Hans J. Stauss Eleni Nastouli Katie J. Doores Marit J. van Gils Laura E. McCoy The effect of spike mutations on SARS-CoV-2 neutralization Cell Reports SARS-CoV-2 neutralization antibodies serology immune escape variant |
| author_facet |
Chloe Rees-Spear Luke Muir Sarah A. Griffith Judith Heaney Yoann Aldon Jonne L. Snitselaar Peter Thomas Carl Graham Jeffrey Seow Nayung Lee Annachiara Rosa Chloe Roustan Catherine F. Houlihan Rogier W. Sanders Ravindra K. Gupta Peter Cherepanov Hans J. Stauss Eleni Nastouli Katie J. Doores Marit J. van Gils Laura E. McCoy |
| author_sort |
Chloe Rees-Spear |
| title |
The effect of spike mutations on SARS-CoV-2 neutralization |
| title_short |
The effect of spike mutations on SARS-CoV-2 neutralization |
| title_full |
The effect of spike mutations on SARS-CoV-2 neutralization |
| title_fullStr |
The effect of spike mutations on SARS-CoV-2 neutralization |
| title_full_unstemmed |
The effect of spike mutations on SARS-CoV-2 neutralization |
| title_sort |
effect of spike mutations on sars-cov-2 neutralization |
| publisher |
Elsevier |
| series |
Cell Reports |
| issn |
2211-1247 |
| publishDate |
2021-03-01 |
| description |
Summary: Multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines show protective efficacy, which is most likely mediated by neutralizing antibodies recognizing the viral entry protein, spike. Because new SARS-CoV-2 variants are emerging rapidly, as exemplified by the B.1.1.7, B.1.351, and P.1 lineages, it is critical to understand whether antibody responses induced by infection with the original SARS-CoV-2 virus or current vaccines remain effective. In this study, we evaluate neutralization of a series of mutated spike pseudotypes based on divergence from SARS-CoV and then compare neutralization of the B.1.1.7 spike pseudotype and individual mutations. Spike-specific monoclonal antibody neutralization is reduced dramatically; in contrast, polyclonal antibodies from individuals infected in early 2020 remain active against most mutated spike pseudotypes, but potency is reduced in a minority of samples. This work highlights that changes in SARS-CoV-2 spike can alter neutralization sensitivity and underlines the need for effective real-time monitoring of emerging mutations and their effect on vaccine efficacy. |
| topic |
SARS-CoV-2 neutralization antibodies serology immune escape variant |
| url |
http://www.sciencedirect.com/science/article/pii/S2211124721002047 |
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doaj-78e75387f25c4488ad981560e9ef8e932021-03-25T04:28:49ZengElsevierCell Reports2211-12472021-03-013412108890The effect of spike mutations on SARS-CoV-2 neutralizationChloe Rees-Spear0Luke Muir1Sarah A. Griffith2Judith Heaney3Yoann Aldon4Jonne L. Snitselaar5Peter Thomas6Carl Graham7Jeffrey Seow8Nayung Lee9Annachiara Rosa10Chloe Roustan11Catherine F. Houlihan12Rogier W. Sanders13Ravindra K. Gupta14Peter Cherepanov15Hans J. Stauss16Eleni Nastouli17Katie J. Doores18Marit J. van Gils19Laura E. McCoy20Institute of Immunity and Transplantation, Division of Infection and Immunity, University College London, London NW3 2PF, UKInstitute of Immunity and Transplantation, Division of Infection and Immunity, University College London, London NW3 2PF, UKInstitute of Immunity and Transplantation, Division of Infection and Immunity, University College London, London NW3 2PF, UKAdvanced Pathogens Diagnostic Unit, Department of Clinical Virology, University College London Hospitals NHS Foundation Trust, London W1T 4EU, UKAmsterdam University Medical Centers, Amsterdam Institute for Infection and Immunity, University of Amsterdam, 1105 AZ Amsterdam, the NetherlandsAmsterdam University Medical Centers, Amsterdam Institute for Infection and Immunity, University of Amsterdam, 1105 AZ Amsterdam, the NetherlandsInstitute of Immunity and Transplantation, Division of Infection and Immunity, University College London, London NW3 2PF, UKSchool of Immunology and Microbial Sciences, King’s College London, London SE1 9RT, UKSchool of Immunology and Microbial Sciences, King’s College London, London SE1 9RT, UKInstitute of Immunity and Transplantation, Division of Infection and Immunity, University College London, London NW3 2PF, UKThe Francis Crick Institute, London NW1 1AT, UKThe Francis Crick Institute, London NW1 1AT, UKAdvanced Pathogens Diagnostic Unit, Department of Clinical Virology, University College London Hospitals NHS Foundation Trust, London W1T 4EU, UK; Research Department of Infection, Division of Infection and Immunity, University College London, London WC1 6BT, UKAmsterdam University Medical Centers, Amsterdam Institute for Infection and Immunity, University of Amsterdam, 1105 AZ Amsterdam, the NetherlandsDepartment of Medicine, University of Cambridge, Cambridge CB2 0AW, UKThe Francis Crick Institute, London NW1 1AT, UKInstitute of Immunity and Transplantation, Division of Infection and Immunity, University College London, London NW3 2PF, UKAdvanced Pathogens Diagnostic Unit, Department of Clinical Virology, University College London Hospitals NHS Foundation Trust, London W1T 4EU, UK; The Francis Crick Institute, London NW1 1AT, UK; Great Ormond Street Institute for Child Health, Infection, Immunity and Inflammation, University College London, London WC1N 1EH, UKSchool of Immunology and Microbial Sciences, King’s College London, London SE1 9RT, UKAmsterdam University Medical Centers, Amsterdam Institute for Infection and Immunity, University of Amsterdam, 1105 AZ Amsterdam, the NetherlandsInstitute of Immunity and Transplantation, Division of Infection and Immunity, University College London, London NW3 2PF, UK; Corresponding authorSummary: Multiple severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccines show protective efficacy, which is most likely mediated by neutralizing antibodies recognizing the viral entry protein, spike. Because new SARS-CoV-2 variants are emerging rapidly, as exemplified by the B.1.1.7, B.1.351, and P.1 lineages, it is critical to understand whether antibody responses induced by infection with the original SARS-CoV-2 virus or current vaccines remain effective. In this study, we evaluate neutralization of a series of mutated spike pseudotypes based on divergence from SARS-CoV and then compare neutralization of the B.1.1.7 spike pseudotype and individual mutations. Spike-specific monoclonal antibody neutralization is reduced dramatically; in contrast, polyclonal antibodies from individuals infected in early 2020 remain active against most mutated spike pseudotypes, but potency is reduced in a minority of samples. This work highlights that changes in SARS-CoV-2 spike can alter neutralization sensitivity and underlines the need for effective real-time monitoring of emerging mutations and their effect on vaccine efficacy.http://www.sciencedirect.com/science/article/pii/S2211124721002047SARS-CoV-2neutralizationantibodiesserologyimmune escapevariant |