Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria

Measuring CPT-1-mediated respiration in permeabilized cells and isolated mitochondria

Summary: Carnitine palmitoyltransferase-1 (CPT-1) is a rate-controlling enzyme for long-chain fatty acid oxidation. This manuscript provides protocols for measuring CPT-1-mediated respiration in permeabilized, adherent cell monolayers and mitochondria freshly isolated from tissue, along with example...

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Bibliographic Details
Main Authors: Krista Yang, Mary T. Doan, Linsey Stiles, Ajit S. Divakaruni
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Cell-based Assays
Cell separation/fractionation
Metabolism
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721003944

Internet

http://www.sciencedirect.com/science/article/pii/S2666166721003944

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