Improvement of IFNg ELISPOT Performance Following Overnight Resting of Frozen PBMC Samples Confirmed Through Rigorous Statistical Analysis

Improvement of IFNg ELISPOT Performance Following Overnight Resting of Frozen PBMC Samples Confirmed Through Rigorous Statistical Analysis

Immune monitoring of functional responses is a fundamental parameter to establish correlates of protection in clinical trials evaluating vaccines and therapies to boost antigen-specific responses. The IFNg ELISPOT assay is a well-standardized and validated method for the determination of functional...

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Bibliographic Details
Main Authors: Radleigh Santos, Alcinette Buying, Nazila Sabri, John Yu, Anthony Gringeri, James Bender, Sylvia Janetzki, Clemencia Pinilla, Valeria A. Judkowski
Format: Article
Language:English
Published: MDPI AG 2014-12-01
Series:Cells
Subjects:
overnight resting
PBMC
IFNg ELISPOT
immune monitoring
Online Access:http://www.mdpi.com/2073-4409/4/1/1

Internet

http://www.mdpi.com/2073-4409/4/1/1

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