Development of an immunoblot assay with infrared fluorescence to quantify paraoxonase 1 in serum and plasma

Paraoxonase 1 (PON1) requires calcium for activity and is inactivated in the presence of EDTA. Because of this, studies to date have used serum or heparinized plasma for both activity and mass assays of PON1. Whole serum and EDTA plasma were analyzed by SDS-electrophoresis and Western blot using ant...

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Bibliographic Details
Main Authors: Philip W. Connelly, Graham F. Maguire, Clive M. Picardo, John F. Teiber, Dragomir Draganov
Format: Article
Language:English
Published: Elsevier 2008-01-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520428986