Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media

Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media

Summary: We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and pri...

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Main Authors: Maciej Daniszewski, Quan Nguyen, Hun S. Chy, Vikrant Singh, Duncan E. Crombie, Tejal Kulkarni, Helena H. Liang, Priyadharshini Sivakumaran, Grace E. Lidgerwood, Damián Hernández, Alison Conquest, Louise A. Rooney, Sophie Chevalier, Stacey B. Andersen, Anne Senabouth, James C. Vickers, David A. Mackey, Jamie E. Craig, Andrew L. Laslett, Alex W. Hewitt, Joseph E. Powell, Alice Pébay
Format: Article
Language:English
Published: Elsevier 2018-09-01
Series:iScience
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004218301287

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http://www.sciencedirect.com/science/article/pii/S2589004218301287

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