High-Quality Library Preparation for NGS-Based Immunoglobulin Germline Gene Inference and Repertoire Expression Analysis

High-Quality Library Preparation for NGS-Based Immunoglobulin Germline Gene Inference and Repertoire Expression Analysis

Next generation sequencing (NGS) of immunoglobulin (Ig) repertoires (Rep-seq) enables examination of the adaptive immune system at an unprecedented level. Applications include studies of expressed repertoires, gene usage, somatic hypermutation levels, Ig lineage tracing and identification of genetic...

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Bibliographic Details
Main Authors: Néstor Vázquez Bernat, Martin Corcoran, Uta Hardt, Mateusz Kaduk, Ganesh E. Phad, Marcel Martin, Gunilla B. Karlsson Hedestam
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Immunology
Subjects:
next generation sequencing
immunoglobulin
antibody
repertoire
library
germline gene
Online Access:https://www.frontiersin.org/article/10.3389/fimmu.2019.00660/full

Internet

https://www.frontiersin.org/article/10.3389/fimmu.2019.00660/full

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