Enzymatic assessment of cholesterol on electrophoresis gels for estimating HDL size distribution and plasma concentrations of HDL subclasses[S]

Enzymatic assessment of cholesterol on electrophoresis gels for estimating HDL size distribution and plasma concentrations of HDL subclasses[S]

The aim of this study was to develop an enzymatic cholesterol staining method to determine HDL subclasses in a polyacrylamide gradient gel electrophoresis, which further allows staining by protein in the same electrophoresis lane. HDLs from 120 healthy individuals were separated through nondenaturin...

Full description

Bibliographic Details
Main Authors: Paola Toledo-Ibelles, Cynthia García-Sánchez, Nydia Ávila-Vazzini, Elizabeth Carreón-Torres, Carlos Posadas-Romero, Gilberto Vargas-Alarcón, Oscar Pérez-Méndez
Format: Article
Language:English
Published: Elsevier 2010-06-01
Series:Journal of Lipid Research
Subjects:
high-density lipoprotein subclasses
enzymatic staining
optical densitometry analysis
HDL composition
cardiovascular risk
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520410363

Internet

http://www.sciencedirect.com/science/article/pii/S0022227520410363

Similar Items