Using the <it>cre-lox</it> recombination system to assess functional impairment caused by amino acid substitutions in yeast proteins

Using the <it>cre-lox</it> recombination system to assess functional impairment caused by amino acid substitutions in yeast proteins

<p>A method was developed to assess the functional significance of a sequence motif in yeast Upf3p, a protein required for nonsense-mediated mRNA decay (NMD). The motif lies at the edge of the Upf3p-Upf2p interaction domain, but at the same time resembles the canonical leucine-rich nuclear exp...

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Bibliographic Details
Main Authors: Shirley Renee L., Richards M. Rachel, Culbertson Michael R.
Format: Article
Language:English
Published: BMC 2004-01-01
Series:Biological Procedures Online
Subjects:
Recombination, Genetic
Gene Expression Regulation, Fungal
RNA Processing, Post-Transcriptional
Online Access:http://www.biologicalprocedures.com/bpo/arts/1/91/m91.htm

Internet

http://www.biologicalprocedures.com/bpo/arts/1/91/m91.htm

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