Using the <it>cre-lox</it> recombination system to assess functional impairment caused by amino acid substitutions in yeast proteins
<p>A method was developed to assess the functional significance of a sequence motif in yeast Upf3p, a protein required for nonsense-mediated mRNA decay (NMD). The motif lies at the edge of the Upf3p-Upf2p interaction domain, but at the same time resembles the canonical leucine-rich nuclear exp...
Main Authors: | Shirley Renee L., Richards M. Rachel, Culbertson Michael R. |
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Format: | Article |
Language: | English |
Published: |
BMC
2004-01-01
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Series: | Biological Procedures Online |
Subjects: | |
Online Access: | http://www.biologicalprocedures.com/bpo/arts/1/91/m91.htm |
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