Sensitive and Rapid Phenotyping of Microbes With Soluble Methane Monooxygenase Using a Droplet-Based Assay

Sensitive and Rapid Phenotyping of Microbes With Soluble Methane Monooxygenase Using a Droplet-Based Assay

Methanotrophs with soluble methane monooxygenase (sMMO) show high potential for various ecological and biotechnological applications. Here, we developed a high throughput method to identify sMMO-producing microbes by integrating droplet microfluidics and a genetic circuit-based biosensor system. sMM...

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Bibliographic Details
Main Authors: Hyewon Lee, Ji In Baek, Su Jin Kim, Kil Koang Kwon, Eugene Rha, Soo-Jin Yeom, Haseong Kim, Dae-Hee Lee, Dong-Myung Kim, Seung-Goo Lee
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-04-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
synthetic biology
transcriptional factor-based biosensors
microfluidics
large-scale phenotyping
cell–cell communication
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.00358/full

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https://www.frontiersin.org/article/10.3389/fbioe.2020.00358/full

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