Etoricoxib decreases subchondral bone mass and attenuates biomechanical properties at the early stage of osteoarthritis in a mouse model
Etoricoxib, a selective Cyclooxygenase-2 (COX-2) inhibitor, is commonly used in osteoarthritis (OA) for pain relief, however, little is known about the effects on subchondral bone. In the current study, OA was induced via destabilization of the medial meniscus (DMM) in C57BL/6 mice. Two days after s...
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doaj-7b026d04ac784a99a6f4e574ca12258a2021-05-20T07:41:38ZengElsevierBiomedicine & Pharmacotherapy0753-33222020-07-01127110144Etoricoxib decreases subchondral bone mass and attenuates biomechanical properties at the early stage of osteoarthritis in a mouse modelBo Liu0Chenchen Ji1Yijie Shao2Ting Liang3Jiaheng He4Huaye Jiang5Guangdong Chen6Zongping Luo7Department of Orthopedics, The First Affiliated Hospital of Soochow University, 899 Pinghai Road, Suzhou, 215006, Jiangsu, PR China; Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, 215006, Jiangsu, PR ChinaDepartment of Orthopedics, The First Affiliated Hospital of Soochow University, 899 Pinghai Road, Suzhou, 215006, Jiangsu, PR China; Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, 215006, Jiangsu, PR ChinaDepartment of Orthopedics, The First Affiliated Hospital of Soochow University, 899 Pinghai Road, Suzhou, 215006, Jiangsu, PR ChinaOrthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, 215006, Jiangsu, PR ChinaDepartment of Orthopedics, The First Affiliated Hospital of Soochow University, 899 Pinghai Road, Suzhou, 215006, Jiangsu, PR China; Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, 215006, Jiangsu, PR ChinaDepartment of Orthopedics, The First Affiliated Hospital of Soochow University, 899 Pinghai Road, Suzhou, 215006, Jiangsu, PR China; Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, 215006, Jiangsu, PR ChinaDepartment of Orthopedics, The First Affiliated Hospital of Soochow University, 899 Pinghai Road, Suzhou, 215006, Jiangsu, PR China; Corresponding author.Department of Orthopedics, The First Affiliated Hospital of Soochow University, 899 Pinghai Road, Suzhou, 215006, Jiangsu, PR China; Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, 215006, Jiangsu, PR China; Corresponding author at: Department of Orthopedics, the First Affiliated Hospital of Soochow University, 899 Pinghai Road, Suzhou, 215006, Jiangsu, PR China and Orthopedic Institute, Soochow University, 708 Renmin Rd, Suzhou, 215006, Jiangsu, PR China.Etoricoxib, a selective Cyclooxygenase-2 (COX-2) inhibitor, is commonly used in osteoarthritis (OA) for pain relief, however, little is known about the effects on subchondral bone. In the current study, OA was induced via destabilization of the medial meniscus (DMM) in C57BL/6 mice. Two days after surgery, mice were treated with different concentrations of Etoricoxib. Four weeks after treatment, micro computed tomography (Micro-CT) analysis, histological analysis, atomic force microscopy (AFM) analysis, and scanning electron microscopy (SEM) were performed to evaluate OA progression. We demonstrated that Etoricoxib inhibited osteophyte formation in the subchondral bone. However, it also reduced the bone volume fraction (BV/TV), lowered trabecular thickness (Tb.Th), and more microfractures and pores were observed in the subchondral bone. Moreover, Etoricoxib reduced the elastic modulus of subchondral bone. Exposure to Etoricoxib further increased the empty/total osteocyte ratio of the subchondral bone. Etoricoxib did not show significant improvement in articular cartilage destruction and synovial inflammation in early OA. Together, our observations suggested that although Etoricoxib can relieve OA-induced pain and inhibit osteophyte formation in the subchondral bone, it can also change the microstructures and biomechanical properties of subchondral bone, promote subchondral bone loss, and reduce subchondral bone quality in early OA mice.http://www.sciencedirect.com/science/article/pii/S075333222030336XCOX-2Biomechanical propertiesEtoricoxibMicrostructuresOsteoarthritisSubchondral bone |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Bo Liu Chenchen Ji Yijie Shao Ting Liang Jiaheng He Huaye Jiang Guangdong Chen Zongping Luo |
spellingShingle |
Bo Liu Chenchen Ji Yijie Shao Ting Liang Jiaheng He Huaye Jiang Guangdong Chen Zongping Luo Etoricoxib decreases subchondral bone mass and attenuates biomechanical properties at the early stage of osteoarthritis in a mouse model Biomedicine & Pharmacotherapy COX-2 Biomechanical properties Etoricoxib Microstructures Osteoarthritis Subchondral bone |
author_facet |
Bo Liu Chenchen Ji Yijie Shao Ting Liang Jiaheng He Huaye Jiang Guangdong Chen Zongping Luo |
author_sort |
Bo Liu |
title |
Etoricoxib decreases subchondral bone mass and attenuates biomechanical properties at the early stage of osteoarthritis in a mouse model |
title_short |
Etoricoxib decreases subchondral bone mass and attenuates biomechanical properties at the early stage of osteoarthritis in a mouse model |
title_full |
Etoricoxib decreases subchondral bone mass and attenuates biomechanical properties at the early stage of osteoarthritis in a mouse model |
title_fullStr |
Etoricoxib decreases subchondral bone mass and attenuates biomechanical properties at the early stage of osteoarthritis in a mouse model |
title_full_unstemmed |
Etoricoxib decreases subchondral bone mass and attenuates biomechanical properties at the early stage of osteoarthritis in a mouse model |
title_sort |
etoricoxib decreases subchondral bone mass and attenuates biomechanical properties at the early stage of osteoarthritis in a mouse model |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2020-07-01 |
description |
Etoricoxib, a selective Cyclooxygenase-2 (COX-2) inhibitor, is commonly used in osteoarthritis (OA) for pain relief, however, little is known about the effects on subchondral bone. In the current study, OA was induced via destabilization of the medial meniscus (DMM) in C57BL/6 mice. Two days after surgery, mice were treated with different concentrations of Etoricoxib. Four weeks after treatment, micro computed tomography (Micro-CT) analysis, histological analysis, atomic force microscopy (AFM) analysis, and scanning electron microscopy (SEM) were performed to evaluate OA progression. We demonstrated that Etoricoxib inhibited osteophyte formation in the subchondral bone. However, it also reduced the bone volume fraction (BV/TV), lowered trabecular thickness (Tb.Th), and more microfractures and pores were observed in the subchondral bone. Moreover, Etoricoxib reduced the elastic modulus of subchondral bone. Exposure to Etoricoxib further increased the empty/total osteocyte ratio of the subchondral bone. Etoricoxib did not show significant improvement in articular cartilage destruction and synovial inflammation in early OA. Together, our observations suggested that although Etoricoxib can relieve OA-induced pain and inhibit osteophyte formation in the subchondral bone, it can also change the microstructures and biomechanical properties of subchondral bone, promote subchondral bone loss, and reduce subchondral bone quality in early OA mice. |
topic |
COX-2 Biomechanical properties Etoricoxib Microstructures Osteoarthritis Subchondral bone |
url |
http://www.sciencedirect.com/science/article/pii/S075333222030336X |
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