Pervasive read-through transcription of T-DNAs is frequent in tobacco BY-2 cells and can effectively induce silencing
Abstract Background Plant transformation via Agrobacterium tumefaciens is characterized by integration of commonly low number of T-DNAs at random positions in the genome. When integrated into an active gene region, promoterless reporter genes placed near the T-DNA border sequence are frequently tran...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
BMC
2018-10-01
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Series: | BMC Plant Biology |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12870-018-1482-3 |