Pervasive read-through transcription of T-DNAs is frequent in tobacco BY-2 cells and can effectively induce silencing

Pervasive read-through transcription of T-DNAs is frequent in tobacco BY-2 cells and can effectively induce silencing

Abstract Background Plant transformation via Agrobacterium tumefaciens is characterized by integration of commonly low number of T-DNAs at random positions in the genome. When integrated into an active gene region, promoterless reporter genes placed near the T-DNA border sequence are frequently tran...

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Bibliographic Details
Main Authors: Vojtěch Čermák, Lukáš Fischer
Format: Article
Language:English
Published: BMC 2018-10-01
Series:BMC Plant Biology
Subjects:
GFP
Inverted repeat
Promoterless
RNAi
Read-through transcription
T-DNA
Online Access:http://link.springer.com/article/10.1186/s12870-018-1482-3

Internet

http://link.springer.com/article/10.1186/s12870-018-1482-3

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