Microwave-Assisted Synthesis of Arene Ru(II) Complexes Induce Tumor Cell Apoptosis Through Selectively Binding and Stabilizing bcl-2 G-Quadruplex DNA
A series of arene Ru(II) complexes coordinated with phenanthroimidazole derivatives, [(η6-C6H6)Ru(l)Cl]Cl(1b L = p-ClPIP = 2-(4-Chlorophenyl)imidazole[4,5f] 1,10-phenanthroline; 2b L = m-ClPIP = 2-(3-Chlorophenyl)imidazole[4,5f] 1,10-phenanthroline; 3b L = p-NPIP = 2-(4-Nitrophenyl)imidazole[4,5f] 1...
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doaj-7b66579aef6142178e87c47977324cdf2020-11-24T22:38:00ZengMDPI AGMaterials1996-19442016-05-019538610.3390/ma9050386ma9050386Microwave-Assisted Synthesis of Arene Ru(II) Complexes Induce Tumor Cell Apoptosis Through Selectively Binding and Stabilizing bcl-2 G-Quadruplex DNAYanhua Chen0Qiong Wu1Xicheng Wang2Qiang Xie3Yunyun Tang4Yutao Lan5Shuangyan Zhang6Wenjie Mei7School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaThe First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, ChinaThe Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 440100, ChinaThe First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou 510080, ChinaSchool of Nursing, Guangdong Pharmaceutical University, Guangzhou 510310, ChinaSchool of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaSchool of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, ChinaA series of arene Ru(II) complexes coordinated with phenanthroimidazole derivatives, [(η6-C6H6)Ru(l)Cl]Cl(1b L = p-ClPIP = 2-(4-Chlorophenyl)imidazole[4,5f] 1,10-phenanthroline; 2b L = m-ClPIP = 2-(3-Chlorophenyl)imidazole[4,5f] 1,10-phenanthroline; 3b L = p-NPIP = 2-(4-Nitrophenyl)imidazole[4,5f] 1,10-phenanthroline; 4b L = m-NPIP = 2-(3-Nitrophenyl) imidazole [4,5f] 1,10-phenanthroline) were synthesized in yields of 89.9%–92.7% under conditions of microwave irradiation heating for 30 min to liberate four arene Ru(II) complexes (1b, 2b, 3b, 4b). The anti-tumor activity of 1b against various tumor cells was evaluated by MTT assay. The results indicated that this complex blocked the growth of human lung adenocarcinoma A549 cells with an IC50 of 16.59 μM. Flow cytometric analysis showed that apoptosis of A549 cells was observed following treatment with 1b. Furthermore, the in vitro DNA-binding behaviors that were confirmed by spectroscopy indicated that 1b could selectively bind and stabilize bcl-2 G-quadruplex DNA to induce apoptosis of A549 cells. Therefore, the synthesized 1b has impressive bcl-2 G-quadruplex DNA-binding and stabilizing activities with potential applications in cancer chemotherapy.http://www.mdpi.com/1996-1944/9/5/386arene Ru(II) complexesmicrowave-assisted synthesisapoptosis inducerselective stabilizingbcl-2 G-quadruplex DNA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yanhua Chen Qiong Wu Xicheng Wang Qiang Xie Yunyun Tang Yutao Lan Shuangyan Zhang Wenjie Mei |
spellingShingle |
Yanhua Chen Qiong Wu Xicheng Wang Qiang Xie Yunyun Tang Yutao Lan Shuangyan Zhang Wenjie Mei Microwave-Assisted Synthesis of Arene Ru(II) Complexes Induce Tumor Cell Apoptosis Through Selectively Binding and Stabilizing bcl-2 G-Quadruplex DNA Materials arene Ru(II) complexes microwave-assisted synthesis apoptosis inducer selective stabilizing bcl-2 G-quadruplex DNA |
author_facet |
Yanhua Chen Qiong Wu Xicheng Wang Qiang Xie Yunyun Tang Yutao Lan Shuangyan Zhang Wenjie Mei |
author_sort |
Yanhua Chen |
title |
Microwave-Assisted Synthesis of Arene Ru(II) Complexes Induce Tumor Cell Apoptosis Through Selectively Binding and Stabilizing bcl-2 G-Quadruplex DNA |
title_short |
Microwave-Assisted Synthesis of Arene Ru(II) Complexes Induce Tumor Cell Apoptosis Through Selectively Binding and Stabilizing bcl-2 G-Quadruplex DNA |
title_full |
Microwave-Assisted Synthesis of Arene Ru(II) Complexes Induce Tumor Cell Apoptosis Through Selectively Binding and Stabilizing bcl-2 G-Quadruplex DNA |
title_fullStr |
Microwave-Assisted Synthesis of Arene Ru(II) Complexes Induce Tumor Cell Apoptosis Through Selectively Binding and Stabilizing bcl-2 G-Quadruplex DNA |
title_full_unstemmed |
Microwave-Assisted Synthesis of Arene Ru(II) Complexes Induce Tumor Cell Apoptosis Through Selectively Binding and Stabilizing bcl-2 G-Quadruplex DNA |
title_sort |
microwave-assisted synthesis of arene ru(ii) complexes induce tumor cell apoptosis through selectively binding and stabilizing bcl-2 g-quadruplex dna |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2016-05-01 |
description |
A series of arene Ru(II) complexes coordinated with phenanthroimidazole derivatives, [(η6-C6H6)Ru(l)Cl]Cl(1b L = p-ClPIP = 2-(4-Chlorophenyl)imidazole[4,5f] 1,10-phenanthroline; 2b L = m-ClPIP = 2-(3-Chlorophenyl)imidazole[4,5f] 1,10-phenanthroline; 3b L = p-NPIP = 2-(4-Nitrophenyl)imidazole[4,5f] 1,10-phenanthroline; 4b L = m-NPIP = 2-(3-Nitrophenyl) imidazole [4,5f] 1,10-phenanthroline) were synthesized in yields of 89.9%–92.7% under conditions of microwave irradiation heating for 30 min to liberate four arene Ru(II) complexes (1b, 2b, 3b, 4b). The anti-tumor activity of 1b against various tumor cells was evaluated by MTT assay. The results indicated that this complex blocked the growth of human lung adenocarcinoma A549 cells with an IC50 of 16.59 μM. Flow cytometric analysis showed that apoptosis of A549 cells was observed following treatment with 1b. Furthermore, the in vitro DNA-binding behaviors that were confirmed by spectroscopy indicated that 1b could selectively bind and stabilize bcl-2 G-quadruplex DNA to induce apoptosis of A549 cells. Therefore, the synthesized 1b has impressive bcl-2 G-quadruplex DNA-binding and stabilizing activities with potential applications in cancer chemotherapy. |
topic |
arene Ru(II) complexes microwave-assisted synthesis apoptosis inducer selective stabilizing bcl-2 G-quadruplex DNA |
url |
http://www.mdpi.com/1996-1944/9/5/386 |
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