Dose‐dependent effects of kefir on colitis induced by trinitrobenzene sulfonic acid in rats
Abstract Evidence suggests that gut microbiota dysbiosis plays a critical role in the initiation and promotion of inflammatory bowel disease (IBD). Kefir is a fermented dairy product including yeast and bacterial species. We aimed to investigate the effect of kefir on trinitrobenzene sulfonic acid (...
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doaj-7b794992fc9d48e3bb7e650279016c502020-11-24T22:04:46ZengWileyFood Science & Nutrition2048-71772019-09-01793110311810.1002/fsn3.1174Dose‐dependent effects of kefir on colitis induced by trinitrobenzene sulfonic acid in ratsNurhayat Ozkan Sevencan0Mehmet Isler1Fatma Nilgun Kapucuoglu2Altug Senol3Burcak Kayhan4Sefa Kiztanir5Muhammed Cem Kockar6Department of Internal Medicine, Medical Faculty The University of Karabuk Karabuk TurkeyDepartment of Gastroenterology, Medical Faculty The University of Suleyman Demirel Isparta TurkeyDepartment of Pathology, Medical Faculty The University of Suleyman Demirel Isparta TurkeyDepartment of Gastroenterology, Medical Faculty The University of Suleyman Demirel Isparta TurkeyDepartment of Internal Medicine, Medical Faculty The University of Karabuk Karabuk TurkeyDepartment of Internal Medicine, Medical Faculty The University of Suleyman Demirel Isparta TurkeyDepartment of Gastroenterology, Medical Faculty The University of Suleyman Demirel Isparta TurkeyAbstract Evidence suggests that gut microbiota dysbiosis plays a critical role in the initiation and promotion of inflammatory bowel disease (IBD). Kefir is a fermented dairy product including yeast and bacterial species. We aimed to investigate the effect of kefir on trinitrobenzene sulfonic acid (TNBS)‐induced colitis in rats using two different doses. Fifty‐four Wistar rats were divided into six groups. For 14 days, the normal control and colitis control groups were given tap water, kefir10 control, kefir10 colitis, and kefir30 control, and the kefir30 colitis groups were given phosphate‐buffered saline containing 10% or 30% kefir, respectively, instead of tap water. Colitis was induced by intracolonically administrating TNBS in the colitis control, kefir10 colitis, and kefir30 colitis groups. On the 14th day, the rats were sacrificed. The weights and lengths of the colons were measured and macroscopically evaluated, and the distal 10 cm segments were subjected to a histopathological examination. The incidence of bloody stool and diarrhea in the kefir10 colitis group was found to be less than the colitis control and kefir30 colitis groups. The colonic weight/length ratio in the kefir10 colitis group was lower than that in the colitis control and kefir30 colitis groups. We detected that the 10% kefir treatment reduced TNBS‐induced macroscopic colonic damage, while it was exacerbated by the 30% kefir treatment. No significant difference was observed between the colitis groups in terms of microscopic colonic damage scoring. These results indicate that kefir, with a careful dose selection, may be a useful agent in the treatment of IBD.https://doi.org/10.1002/fsn3.1174colitisinflammatory bowel diseasekefirprobiotictrinitrobenzene sulfonic acid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Nurhayat Ozkan Sevencan Mehmet Isler Fatma Nilgun Kapucuoglu Altug Senol Burcak Kayhan Sefa Kiztanir Muhammed Cem Kockar |
spellingShingle |
Nurhayat Ozkan Sevencan Mehmet Isler Fatma Nilgun Kapucuoglu Altug Senol Burcak Kayhan Sefa Kiztanir Muhammed Cem Kockar Dose‐dependent effects of kefir on colitis induced by trinitrobenzene sulfonic acid in rats Food Science & Nutrition colitis inflammatory bowel disease kefir probiotic trinitrobenzene sulfonic acid |
author_facet |
Nurhayat Ozkan Sevencan Mehmet Isler Fatma Nilgun Kapucuoglu Altug Senol Burcak Kayhan Sefa Kiztanir Muhammed Cem Kockar |
author_sort |
Nurhayat Ozkan Sevencan |
title |
Dose‐dependent effects of kefir on colitis induced by trinitrobenzene sulfonic acid in rats |
title_short |
Dose‐dependent effects of kefir on colitis induced by trinitrobenzene sulfonic acid in rats |
title_full |
Dose‐dependent effects of kefir on colitis induced by trinitrobenzene sulfonic acid in rats |
title_fullStr |
Dose‐dependent effects of kefir on colitis induced by trinitrobenzene sulfonic acid in rats |
title_full_unstemmed |
Dose‐dependent effects of kefir on colitis induced by trinitrobenzene sulfonic acid in rats |
title_sort |
dose‐dependent effects of kefir on colitis induced by trinitrobenzene sulfonic acid in rats |
publisher |
Wiley |
series |
Food Science & Nutrition |
issn |
2048-7177 |
publishDate |
2019-09-01 |
description |
Abstract Evidence suggests that gut microbiota dysbiosis plays a critical role in the initiation and promotion of inflammatory bowel disease (IBD). Kefir is a fermented dairy product including yeast and bacterial species. We aimed to investigate the effect of kefir on trinitrobenzene sulfonic acid (TNBS)‐induced colitis in rats using two different doses. Fifty‐four Wistar rats were divided into six groups. For 14 days, the normal control and colitis control groups were given tap water, kefir10 control, kefir10 colitis, and kefir30 control, and the kefir30 colitis groups were given phosphate‐buffered saline containing 10% or 30% kefir, respectively, instead of tap water. Colitis was induced by intracolonically administrating TNBS in the colitis control, kefir10 colitis, and kefir30 colitis groups. On the 14th day, the rats were sacrificed. The weights and lengths of the colons were measured and macroscopically evaluated, and the distal 10 cm segments were subjected to a histopathological examination. The incidence of bloody stool and diarrhea in the kefir10 colitis group was found to be less than the colitis control and kefir30 colitis groups. The colonic weight/length ratio in the kefir10 colitis group was lower than that in the colitis control and kefir30 colitis groups. We detected that the 10% kefir treatment reduced TNBS‐induced macroscopic colonic damage, while it was exacerbated by the 30% kefir treatment. No significant difference was observed between the colitis groups in terms of microscopic colonic damage scoring. These results indicate that kefir, with a careful dose selection, may be a useful agent in the treatment of IBD. |
topic |
colitis inflammatory bowel disease kefir probiotic trinitrobenzene sulfonic acid |
url |
https://doi.org/10.1002/fsn3.1174 |
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