Purification and n-terminal sequencing of two presynaptic neurotoxic PLA2, neuwieditoxin-I and neuwieditoxin-II, from Bothrops neuwiedi pauloensis (jararaca pintada) venom

Purification and n-terminal sequencing of two presynaptic neurotoxic PLA2, neuwieditoxin-I and neuwieditoxin-II, from Bothrops neuwiedi pauloensis (jararaca pintada) venom

Two presynaptic phospholipases A2 (PLA2), neuwieditoxin-I (NeuTX-I) and neuwieditoxin-II (NeuTX-II), were isolated from the venom of Bothrops neuwiedi pauloensis (BNP). The venom was fractionated using molecular exclusion HPLC (Protein-Pak 300SW column), followed by reverse phase HPLC (µBondapak C18...

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Bibliographic Details
Main Authors: C. R. Borja-Oliveira, B. H. Kassab, A. M. Soares, M. H. Toyama, J. R. Giglio, S. Marangoni, L. Re, L. Rodrigues-Simioni
Format: Article
Language:English
Published: SciELO 2007-01-01
Series:Journal of Venomous Animals and Toxins including Tropical Diseases
Subjects:
chick biventer cervicis
loose patch clamp
nerve-muscle preparation
neuromuscular junction
neurotoxicity
PLA2 neurotoxin
presynaptic action
Bothrops neuwiedi pauloensis
Neuwieditoxin-I
Neuwieditoxin-II
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100008

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100008

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