Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase

Native top-down mass spectrometry provides insights into the copper centers of membrane-bound methane monooxygenase

The activity of the membrane-bound enzyme pMMO depends on copper but the location of the copper centers is still under debate. Here, the authors reconstitute pMMO in nanodiscs and use native top-down MS to localize its copper centers, providing insights into which sites are essential for activity.

Bibliographic Details
Main Authors: Soo Y. Ro, Luis F. Schachner, Christopher W. Koo, Rahul Purohit, Jonathan P. Remis, Grace E. Kenney, Brandon W. Liauw, Paul M. Thomas, Steven M. Patrie, Neil L. Kelleher, Amy C. Rosenzweig
Format: Article
Language:English
Published: Nature Publishing Group 2019-06-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-10590-6

Internet

https://doi.org/10.1038/s41467-019-10590-6

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