Functional characterization and stability improvement of a ‘thermophilic-like’ ene-reductase from Rhodococcus opacus 1CP

Functional characterization and stability improvement of a ‘thermophilic-like’ ene-reductase from Rhodococcus opacus 1CP

Ene-reductases are widely applied for the asymmetric synthesis of relevant industrial chemicals. A novel ene-reductase OYERo2 was found within a set of 14 putative Old Yellow Enzymes (OYEs) obtained by genome mining of the actinobacterium Rhodococcus opacus 1CP. Multiple sequence alignment suggested...

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Bibliographic Details
Main Authors: Anika eRiedel, Marika eMehnert, Caroline E. Paul, Adrie H. Westphal, Willem JH Van Berkel, Dirk eTischler
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-10-01
Series:Frontiers in Microbiology
Subjects:
Biocatalysis
flavoprotein
Thermal stability
ene-reductase
Old Yellow Enzyme
Rhodococcus opacus 1CP
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01073/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.01073/full

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