Synthesis of [13C4]-labeled ∆9-Tetrahydrocannabinol and 11-nor-9-Carboxy-∆9-tetrahydrocannabinol as Internal Standards for Reducing Ion Suppressing/Alteration Effects in LC/MS-MS Quantification
(−)-∆9-Tetrahydrocannabinol is the principal psychoactive component of the cannabis plant and also the active ingredient in some prescribed drugs. To detect and control misuse and monitor administration in clinical settings, reference samples of the native drugs and their metabolites are needed. Th...
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doaj-7c071aa83bb440e497c2c5be73607f6c2020-11-24T20:57:07ZengMDPI AGMolecules1420-30492014-09-01199135261354010.3390/molecules190913526molecules190913526Synthesis of [13C4]-labeled ∆9-Tetrahydrocannabinol and 11-nor-9-Carboxy-∆9-tetrahydrocannabinol as Internal Standards for Reducing Ion Suppressing/Alteration Effects in LC/MS-MS QuantificationMorten Karlsen0Huiling Liu1Jon Eigill Johansen2Bård Helge Hoff3Department of Chemistry, Norwegian University of Science and Technology, Høgskoleringen 5, 7491 Trondheim, NorwayChiron AS, Stiklestadveien 1, 7041 Trondheim 7041, NorwayChiron AS, Stiklestadveien 1, 7041 Trondheim 7041, NorwayDepartment of Chemistry, Norwegian University of Science and Technology, Høgskoleringen 5, 7491 Trondheim, Norway(−)-∆9-Tetrahydrocannabinol is the principal psychoactive component of the cannabis plant and also the active ingredient in some prescribed drugs. To detect and control misuse and monitor administration in clinical settings, reference samples of the native drugs and their metabolites are needed. The accuracy of liquid chromatography/mass spectrometric quantification of drugs in biological samples depends among others on ion suppressing/alteration effects. Especially, 13C-labeled drug analogues are useful for minimzing such interferences. Thus, to provide internal standards for more accurate quantification and for identification purpose, synthesis of [13C4]-∆9-tetrahydro-cannabinol and [13C4]-11-nor-9-carboxy-∆9-tetrahydrocannabinol was developed via [13C4]-olivetol. Starting from [13C4]-olivetol the synthesis of [13C4]-11-nor-9-carboxy-∆9-tetrahydrocannabinol was shortened from three to two steps by employing nitromethane as a co-solvent in condensation with (+)-apoverbenone.http://www.mdpi.com/1420-3049/19/9/1352613C-labeled standards[13C4]-olivetol[13C4]-∆9-tetrahydrocannabinol[13C4]-11-nor-9-carboxy-∆9-tetrahydrocannabinol |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Morten Karlsen Huiling Liu Jon Eigill Johansen Bård Helge Hoff |
spellingShingle |
Morten Karlsen Huiling Liu Jon Eigill Johansen Bård Helge Hoff Synthesis of [13C4]-labeled ∆9-Tetrahydrocannabinol and 11-nor-9-Carboxy-∆9-tetrahydrocannabinol as Internal Standards for Reducing Ion Suppressing/Alteration Effects in LC/MS-MS Quantification Molecules 13C-labeled standards [13C4]-olivetol [13C4]-∆9-tetrahydrocannabinol [13C4]-11-nor-9-carboxy-∆9-tetrahydrocannabinol |
author_facet |
Morten Karlsen Huiling Liu Jon Eigill Johansen Bård Helge Hoff |
author_sort |
Morten Karlsen |
title |
Synthesis of [13C4]-labeled ∆9-Tetrahydrocannabinol and 11-nor-9-Carboxy-∆9-tetrahydrocannabinol as Internal Standards for Reducing Ion Suppressing/Alteration Effects in LC/MS-MS Quantification |
title_short |
Synthesis of [13C4]-labeled ∆9-Tetrahydrocannabinol and 11-nor-9-Carboxy-∆9-tetrahydrocannabinol as Internal Standards for Reducing Ion Suppressing/Alteration Effects in LC/MS-MS Quantification |
title_full |
Synthesis of [13C4]-labeled ∆9-Tetrahydrocannabinol and 11-nor-9-Carboxy-∆9-tetrahydrocannabinol as Internal Standards for Reducing Ion Suppressing/Alteration Effects in LC/MS-MS Quantification |
title_fullStr |
Synthesis of [13C4]-labeled ∆9-Tetrahydrocannabinol and 11-nor-9-Carboxy-∆9-tetrahydrocannabinol as Internal Standards for Reducing Ion Suppressing/Alteration Effects in LC/MS-MS Quantification |
title_full_unstemmed |
Synthesis of [13C4]-labeled ∆9-Tetrahydrocannabinol and 11-nor-9-Carboxy-∆9-tetrahydrocannabinol as Internal Standards for Reducing Ion Suppressing/Alteration Effects in LC/MS-MS Quantification |
title_sort |
synthesis of [13c4]-labeled ∆9-tetrahydrocannabinol and 11-nor-9-carboxy-∆9-tetrahydrocannabinol as internal standards for reducing ion suppressing/alteration effects in lc/ms-ms quantification |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2014-09-01 |
description |
(−)-∆9-Tetrahydrocannabinol is the principal psychoactive component of the cannabis plant and also the active ingredient in some prescribed drugs. To detect and control misuse and monitor administration in clinical settings, reference samples of the native drugs and their metabolites are needed. The accuracy of liquid chromatography/mass spectrometric quantification of drugs in biological samples depends among others on ion suppressing/alteration effects. Especially, 13C-labeled drug analogues are useful for minimzing such interferences. Thus, to provide internal standards for more accurate quantification and for identification purpose, synthesis of [13C4]-∆9-tetrahydro-cannabinol and [13C4]-11-nor-9-carboxy-∆9-tetrahydrocannabinol was developed via [13C4]-olivetol. Starting from [13C4]-olivetol the synthesis of [13C4]-11-nor-9-carboxy-∆9-tetrahydrocannabinol was shortened from three to two steps by employing nitromethane as a co-solvent in condensation with (+)-apoverbenone. |
topic |
13C-labeled standards [13C4]-olivetol [13C4]-∆9-tetrahydrocannabinol [13C4]-11-nor-9-carboxy-∆9-tetrahydrocannabinol |
url |
http://www.mdpi.com/1420-3049/19/9/13526 |
work_keys_str_mv |
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1716788810107322368 |