Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping

Abstract Background Direct molecular cloning of full-length cDNAs derived from viral RNA is an approach to identify the individual viral genomes within a virus population. This enables characterization of distinct viral haplotypes present during infection. Results In this study, we recover individua...

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Bibliographic Details
Main Authors: Camille Melissa Johnston, Ulrik Fahnøe, Graham J. Belsham, Thomas Bruun Rasmussen
Format: Article
Language:English
Published: BMC 2018-08-01
Series:BMC Genomics
Subjects:
RNA
Online Access:http://link.springer.com/article/10.1186/s12864-018-4971-8