A novel air-dried multiplex high-resolution melt assay for the detection of extended-spectrum β-lactamase and carbapenemase genes

A novel air-dried multiplex high-resolution melt assay for the detection of extended-spectrum β-lactamase and carbapenemase genes

ABSTRACT: Objectives: This study aimed to develop and evaluate a novel air-dried high-resolution melt (HRM) assay to detect eight major extended-spectrum β-lactamase (ESBL) (blaSHV and blaCTX-M groups 1 and 9) and carbapenemase (blaNDM, blaIMP, blaKPC, blaVIM and blaOXA-48-like) genes that confer r...

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Bibliographic Details
Main Authors: Ana I. Cubas-Atienzar, Christopher T. Williams, Abhilasha Karkey, Sabina Dongol, Manandhar Sulochana, Shrestha Rajendra, Glyn Hobbs, Katie Evans, Patrick Musicha, Nicholas Feasey, Luis E. Cuevas, Emily R. Adams, Thomas Edwards
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:Journal of Global Antimicrobial Resistance
Subjects:
Antimicrobial resistance
Extended-spectrum β-lactamase
ESBL
Carbapenemase
High-resolution melting
Molecular diagnostics
Online Access:http://www.sciencedirect.com/science/article/pii/S2213716521001971

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http://www.sciencedirect.com/science/article/pii/S2213716521001971

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