Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice
Abstract Tuberculosis, caused by Mycobacterium tuberculosis, affects the functions of the lung and causes high morbidity and mortality rates worldwide. MASP-2 is an executioner enzyme, which plays an essential role in the activation of lectin pathway. In our previous studies, the MASP-2 played a dua...
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doaj-7c1627ec235349c68cda3d9447c015412020-12-08T01:51:49ZengNature Publishing GroupScientific Reports2045-23222017-05-017111110.1038/s41598-017-02374-zEffects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c miceXiaoying Xu0Xiaoling Lu1Xingfang Dong2Yanping Luo3Qian Wang4Xun Liu5Jie Fu6Yuan Zhang7Bingdong Zhu8Xingming Ma9Department of Immunology, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversityInstitute of Pathogenic Biology, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversityInstitute of Pathogenic Biology, School of Basic Medical Sciences, Lanzhou UniversityDepartment of Immunology, School of Basic Medical Sciences, Lanzhou UniversityAbstract Tuberculosis, caused by Mycobacterium tuberculosis, affects the functions of the lung and causes high morbidity and mortality rates worldwide. MASP-2 is an executioner enzyme, which plays an essential role in the activation of lectin pathway. In our previous studies, the MASP-2 played a dual role in promoting the progress of lesions in BCG-infected rabbit skin models. However, the really effects of MASP-2 on tuberculosis are unknown. The aim of this study was to investigate the effects of MASP-2 in granuloma formation with BCG-infected mice. Compared to the control group, rAd-hMASP-2 treated group showed increasing in survival rate of BCG-infected mice (P = 0.042), and decreasing of bacteria loads (P = 0.005) in the lung tissue. MASP-2 displayed a protective efficacy in BCG-infected mice, which promoted the activation and recruitment of macrophages and lymphocytes to the granuloma. Moreover, the data obtained from the ELISA and RT-PCR demonstrated that mRNA expression for IL-6, CCL12, CCL2 and cytokines of IFN-γ, TNF-α in lung were significantly elevated by treatment of rAd-hMASP-2. Those findings provided an evidence that MASP-2 may be as a newly immunomodulatory in targeting granuloma formation, which displayed a potential protective role in control of tuberculosis.https://doi.org/10.1038/s41598-017-02374-z |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaoying Xu Xiaoling Lu Xingfang Dong Yanping Luo Qian Wang Xun Liu Jie Fu Yuan Zhang Bingdong Zhu Xingming Ma |
spellingShingle |
Xiaoying Xu Xiaoling Lu Xingfang Dong Yanping Luo Qian Wang Xun Liu Jie Fu Yuan Zhang Bingdong Zhu Xingming Ma Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice Scientific Reports |
author_facet |
Xiaoying Xu Xiaoling Lu Xingfang Dong Yanping Luo Qian Wang Xun Liu Jie Fu Yuan Zhang Bingdong Zhu Xingming Ma |
author_sort |
Xiaoying Xu |
title |
Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice |
title_short |
Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice |
title_full |
Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice |
title_fullStr |
Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice |
title_full_unstemmed |
Effects of hMASP-2 on the formation of BCG infection-induced granuloma in the lungs of BALB/c mice |
title_sort |
effects of hmasp-2 on the formation of bcg infection-induced granuloma in the lungs of balb/c mice |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-05-01 |
description |
Abstract Tuberculosis, caused by Mycobacterium tuberculosis, affects the functions of the lung and causes high morbidity and mortality rates worldwide. MASP-2 is an executioner enzyme, which plays an essential role in the activation of lectin pathway. In our previous studies, the MASP-2 played a dual role in promoting the progress of lesions in BCG-infected rabbit skin models. However, the really effects of MASP-2 on tuberculosis are unknown. The aim of this study was to investigate the effects of MASP-2 in granuloma formation with BCG-infected mice. Compared to the control group, rAd-hMASP-2 treated group showed increasing in survival rate of BCG-infected mice (P = 0.042), and decreasing of bacteria loads (P = 0.005) in the lung tissue. MASP-2 displayed a protective efficacy in BCG-infected mice, which promoted the activation and recruitment of macrophages and lymphocytes to the granuloma. Moreover, the data obtained from the ELISA and RT-PCR demonstrated that mRNA expression for IL-6, CCL12, CCL2 and cytokines of IFN-γ, TNF-α in lung were significantly elevated by treatment of rAd-hMASP-2. Those findings provided an evidence that MASP-2 may be as a newly immunomodulatory in targeting granuloma formation, which displayed a potential protective role in control of tuberculosis. |
url |
https://doi.org/10.1038/s41598-017-02374-z |
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