Technical advance in targeted NGS analysis enables identification of lung cancer risk-associated low frequency TP53, PIK3CA, and BRAF mutations in airway epithelial cells

Technical advance in targeted NGS analysis enables identification of lung cancer risk-associated low frequency TP53, PIK3CA, and BRAF mutations in airway epithelial cells

Abstract Background Standardized Nucleic Acid Quantification for SEQuencing (SNAQ-SEQ) is a novel method that utilizes synthetic DNA internal standards spiked into each sample prior to next generation sequencing (NGS) library preparation. This method was applied to analysis of normal appearing airwa...

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Bibliographic Details
Main Authors: Daniel J. Craig, Thomas Morrison, Sadik A. Khuder, Erin L. Crawford, Leihong Wu, Joshua Xu, Thomas M. Blomquist, James C. Willey
Format: Article
Language:English
Published: BMC 2019-11-01
Series:BMC Cancer
Subjects:
Biomarker
Low-frequency variant detection
Next generation sequencing
Lung Cancer
Online Access:http://link.springer.com/article/10.1186/s12885-019-6313-x

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http://link.springer.com/article/10.1186/s12885-019-6313-x

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