Integrative structural biology studies of HIV-1 reverse transcriptase binding to a high-affinity DNA aptamer

Integrative structural biology studies of HIV-1 reverse transcriptase binding to a high-affinity DNA aptamer

The high-resolution crystal structure of HIV-1 reverse transcriptase (RT) bound to a 38-mer DNA hairpin aptamer with low pM affinity was previously described. The high-affinity binding aptamer contained 2′-O-methyl modifications and a seven base-pair GC-rich tract and the structure of the RT-aptamer...

Full description

Bibliographic Details
Main Authors: Steve Tuske, Jie Zheng, Erik D. Olson, Francesc X. Ruiz, Bruce D. Pascal, Anthony Hoang, Joseph D. Bauman, Kalyan Das, Jeffrey J. DeStefano, Karin Musier-Forsyth, Patrick R. Griffin, Eddy Arnold
Format: Article
Language:English
Published: Elsevier 2020-01-01
Series:Current Research in Structural Biology
Subjects:
HDX-MS high-Affinity DNA aptamer
HIV-1 reverse transcriptase
Subunit-specific 15N-labeling
SELEX
Integrative structural biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2665928X20300131

Internet

http://www.sciencedirect.com/science/article/pii/S2665928X20300131

Similar Items