Analysis, optimization and verification of Illumina-generated 16S rRNA gene amplicon surveys.

The exploration of microbial communities by sequencing 16S rRNA genes has expanded with low-cost, high-throughput sequencing instruments. Illumina-based 16S rRNA gene sequencing has recently gained popularity over 454 pyrosequencing due to its lower costs, higher accuracy and greater throughput. Alt...

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Bibliographic Details
Main Authors: Michael C Nelson, Hilary G Morrison, Jacquelynn Benjamino, Sharon L Grim, Joerg Graf
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3983156?pdf=render