ssDNA recombineering boosts in vivo evolution of nanobodies displayed on bacterial surfaces
Yamal Al-ramahi et al. describe an accelerated laboratory evolution method that applies ssDNA recombineering and bacterial surface display to engineer nanobody epitope recognition. As a proof-of-concept, they evolved TD4 recognition to bind the translocated intimin receptor (TirM) of EPEC pathogens,...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2021-10-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-021-02702-0 |