The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models
Targeted genome editing technology has been widely used in biomedical studies. The CRISPR-associated RNA-guided endonuclease Cas9 has become a versatile genome editing tool. The CRISPR/Cas9 system is useful for studying gene function through efficient knock-out, knock-in or chromatin modification of...
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Science Press, PR China
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doaj-7d01bc279451475eb2c8d7c813012b812020-11-24T23:57:59ZengScience Press, PR ChinaZoological Research2095-81372095-81372016-07-0137419120410.13918/j.issn.2095-8137.2016.4.191The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal modelsMing SHAO0Tian-Rui XU1Ce-Shi CHEN2Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming Yunnan 650500, China; Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223 ChinaFaculty of Life Science and Technology, Kunming University of Science and Technology, Kunming Yunnan 650500, ChinaKey Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming Yunnan 650223 ChinaTargeted genome editing technology has been widely used in biomedical studies. The CRISPR-associated RNA-guided endonuclease Cas9 has become a versatile genome editing tool. The CRISPR/Cas9 system is useful for studying gene function through efficient knock-out, knock-in or chromatin modification of the targeted gene loci in various cell types and organisms. It can be applied in a number of fields, such as genetic breeding, disease treatment and gene functional investigation. In this review, we introduce the most recent developments and applications, the challenges, and future directions of Cas9 in generating disease animal model. Derived from the CRISPR adaptive immune system of bacteria, the development trend of Cas9 will inevitably fuel the vital applications from basic research to biotechnology and bio-medicine.http://www.zoores.ac.cn/EN/abstract/abstract3703.shtmlCRISPR/Cas9Animal modelsGene therapy |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ming SHAO Tian-Rui XU Ce-Shi CHEN |
spellingShingle |
Ming SHAO Tian-Rui XU Ce-Shi CHEN The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models Zoological Research CRISPR/Cas9 Animal models Gene therapy |
author_facet |
Ming SHAO Tian-Rui XU Ce-Shi CHEN |
author_sort |
Ming SHAO |
title |
The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models |
title_short |
The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models |
title_full |
The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models |
title_fullStr |
The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models |
title_full_unstemmed |
The big bang of genome editing technology: development and application of the CRISPR/Cas9 system in disease animal models |
title_sort |
big bang of genome editing technology: development and application of the crispr/cas9 system in disease animal models |
publisher |
Science Press, PR China |
series |
Zoological Research |
issn |
2095-8137 2095-8137 |
publishDate |
2016-07-01 |
description |
Targeted genome editing technology has been widely used in biomedical studies. The CRISPR-associated RNA-guided endonuclease Cas9 has become a versatile genome editing tool. The CRISPR/Cas9 system is useful for studying gene function through efficient knock-out, knock-in or chromatin modification of the targeted gene loci in various cell types and organisms. It can be applied in a number of fields, such as genetic breeding, disease treatment and gene functional investigation. In this review, we introduce the most recent developments and applications, the challenges, and future directions of Cas9 in generating disease animal model. Derived from the CRISPR adaptive immune system of bacteria, the development trend of Cas9 will inevitably fuel the vital applications from basic research to biotechnology and bio-medicine. |
topic |
CRISPR/Cas9 Animal models Gene therapy |
url |
http://www.zoores.ac.cn/EN/abstract/abstract3703.shtml |
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