Rapid detection and simultaneous genotyping of Cronobacter spp. (formerly Enterobacter sakazakii) in powdered infant formula using real-time PCR and high resolution melting (HRM) analysis.

Rapid detection and simultaneous genotyping of Cronobacter spp. (formerly Enterobacter sakazakii) in powdered infant formula using real-time PCR and high resolution melting (HRM) analysis.

Cronobacter spp. is an emerging pathogen that causes meningitis, sepsis, bacteremia, and necrotizing enterocolitis in neonates and children. The present study developed an assay integrating real-time PCR and high resolution melting (HRM) analysis targeting the OmpA gene for the specific detection an...

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Bibliographic Details
Main Authors: Xian-Quan Cai, Hai-Qiong Yu, Zhou-Xi Ruan, Lei-Liang Yang, Jian-Shan Bai, De-Yi Qiu, Zhi-Hua Jian, Yi-Qian Xiao, Jie-Yang Yang, Thanh Hoa Le, Xing-Quan Zhu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3692429?pdf=render

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http://europepmc.org/articles/PMC3692429?pdf=render

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