The Combined Repetitive Oligopeptides of Clostridium difficile Toxin A Counteract Premature Cleavage of the Glucosyl-Transferase Domain by Stabilizing Protein Conformation

The Combined Repetitive Oligopeptides of Clostridium difficile Toxin A Counteract Premature Cleavage of the Glucosyl-Transferase Domain by Stabilizing Protein Conformation

Toxin A (TcdA) and B (TcdB) from Clostridium difficile enter host cells by receptor-mediated endocytosis. A prerequisite for proper toxin action is the intracellular release of the glucosyltransferase domain by an inherent cysteine protease, which is allosterically activated by inositol hexaphosphat...

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Bibliographic Details
Main Authors: Alexandra Olling, Corinna Hüls, Sebastian Goy, Mirco Müller, Simon Krooss, Isa Rudolf, Helma Tatge, Ralf Gerhard
Format: Article
Language:English
Published: MDPI AG 2014-07-01
Series:Toxins
Subjects:
Clostridium difficile toxin
domain interaction
autoprocessing
cytotoxicity
microscale thermophoresis
Online Access:http://www.mdpi.com/2072-6651/6/7/2162

Internet

http://www.mdpi.com/2072-6651/6/7/2162

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