Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing

Recruitment of DNA Repair MRN Complex by Intrinsically Disordered Protein Domain Fused to Cas9 Improves Efficiency of CRISPR-Mediated Genome Editing

CRISPR/Cas9 is a powerful tool for genome editing in cells and organisms. Nevertheless, introducing directed templated changes by homology-directed repair (HDR) requires the cellular DNA repair machinery, such as the MRN complex (Mre11/Rad50/Nbs1). To improve the process, we tailored chimeric constr...

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Bibliographic Details
Main Authors: Nina Reuven, Julia Adler, Karin Broennimann, Nadav Myers, Yosef Shaul
Format: Article
Language:English
Published: MDPI AG 2019-10-01
Series:Biomolecules
Subjects:
gene targeting
crispr/cas9
homology-directed repair
genome editing
endogenous mutagenesis in cell lines
Online Access:https://www.mdpi.com/2218-273X/9/10/584

Internet

https://www.mdpi.com/2218-273X/9/10/584

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