Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids

Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids

Summary: We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids,...

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Bibliographic Details
Main Authors: Linda Bojmar, Han Sang Kim, Gabriel C. Tobias, Fanny A. Pelissier Vatter, Serena Lucotti, Kofi Ennu Gyan, Candia M. Kenific, Zurong Wan, Kyung-A Kim, DooA Kim, Jonathan Hernandez, Virginia Pascual, Todd E. Heaton, Michael P. La Quaglia, David Kelsen, Tanya M. Trippett, David R. Jones, William R. Jarnagin, Irina R. Matei, Haiying Zhang, Ayuko Hoshino, David Lyden
Format: Article
Language:English
Published: Elsevier 2021-03-01
Series:STAR Protocols
Subjects:
Cell culture
Cell isolation
Cell Membrane
Exosomes
Exomeres
Extracellular vesicles and particles
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720302124

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http://www.sciencedirect.com/science/article/pii/S2666166720302124

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