α-Chaconine Affects the Apoptosis, Mechanical Barrier Function, and Antioxidant Ability of Mouse Small Intestinal Epithelial Cells

α-Chaconine Affects the Apoptosis, Mechanical Barrier Function, and Antioxidant Ability of Mouse Small Intestinal Epithelial Cells

α-Chaconine is the most abundant glycoalkaloid in potato and toxic to the animal digestive system, but the mechanisms underlying the toxicity are unclear. In this study, mouse small intestinal epithelial cells were incubated with α-chaconine at 0, 0.4, and 0.8 μg/mL for 24, 48, and 72 h to examine a...

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Main Authors: Yuhua He, Jiaqi Chen, Qiyue Zhang, Jialong Zhang, Lulai Wang, Xiaoxia Chen, Adrian J. Molenaar, Xuezhao Sun
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-06-01
Series:Frontiers in Plant Science
Subjects:
α-chaconine
intestinal epithelial cell
cell cycle
cell apoptosis
mechanical barrier function
antioxidant ability
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2021.673774/full
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spelling doaj-7dcc0e305f7a4926b3ad3a5144c53f8c2021-06-09T06:21:10ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-06-011210.3389/fpls.2021.673774673774α-Chaconine Affects the Apoptosis, Mechanical Barrier Function, and Antioxidant Ability of Mouse Small Intestinal Epithelial CellsYuhua He0Yuhua He1Yuhua He2Jiaqi Chen3Qiyue Zhang4Jialong Zhang5Lulai Wang6Xiaoxia Chen7Adrian J. Molenaar8Xuezhao Sun9Xuezhao Sun10Xuezhao Sun11College of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin City, ChinaThe Innovation Centre of Ruminant Precision Nutrition and Smart and Ecological Farming, Jilin Agricultural Science and Technology University, Jilin City, ChinaJilin Inter-Regional Cooperation Centre for the Scientific and Technological Innovation of Ruminant Precision Nutrition and Smart and Ecological Farming, Jilin City, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin City, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin City, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin City, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin City, ChinaCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin City, ChinaAgResearch Ltd., Grasslands Research Centre, Palmerston North, New ZealandCollege of Animal Science and Technology, Jilin Agricultural Science and Technology University, Jilin City, ChinaThe Innovation Centre of Ruminant Precision Nutrition and Smart and Ecological Farming, Jilin Agricultural Science and Technology University, Jilin City, ChinaJilin Inter-Regional Cooperation Centre for the Scientific and Technological Innovation of Ruminant Precision Nutrition and Smart and Ecological Farming, Jilin City, Chinaα-Chaconine is the most abundant glycoalkaloid in potato and toxic to the animal digestive system, but the mechanisms underlying the toxicity are unclear. In this study, mouse small intestinal epithelial cells were incubated with α-chaconine at 0, 0.4, and 0.8 μg/mL for 24, 48, and 72 h to examine apoptosis, mechanical barrier function, and antioxidant ability of the cells using a cell metabolic activity assay, flow cytometry, Western blot, immunofluorescence, and fluorescence quantitative PCR. The results showed that α-chaconine significantly decreased cell proliferation rate, increased apoptosis rate, decreased transepithelial electrical resistance (TEER) value, and increased alkaline phosphatase (AKP) and lactate dehydrogenase (LDH) activities, and there were interactions between α-chaconine concentration and incubation time. α-Chaconine significantly reduced the relative and mRNA expressions of genes coding tight junction proteins zonula occludens-1 (ZO-1) and occludin, increased malondialdehyde (MDA) content, decreased total glutathione (T-GSH) content, reduced the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and γ-glutamylcysteine synthetase (γ-GCS) and the mRNA expressions of SOD, CAT, GSH-Px, and γ-GCS genes. In conclusion, α-chaconine disrupts the cell cycle, destroys the mechanical barrier and permeability of mucosal epithelium, inhibits cell proliferation, and accelerates cell apoptosis.https://www.frontiersin.org/articles/10.3389/fpls.2021.673774/fullα-chaconineintestinal epithelial cellcell cyclecell apoptosismechanical barrier functionantioxidant ability
collection DOAJ
language English
format Article
sources DOAJ
author Yuhua He
Yuhua He
Yuhua He
Jiaqi Chen
Qiyue Zhang
Jialong Zhang
Lulai Wang
Xiaoxia Chen
Adrian J. Molenaar
Xuezhao Sun
Xuezhao Sun
Xuezhao Sun
spellingShingle Yuhua He
Yuhua He
Yuhua He
Jiaqi Chen
Qiyue Zhang
Jialong Zhang
Lulai Wang
Xiaoxia Chen
Adrian J. Molenaar
Xuezhao Sun
Xuezhao Sun
Xuezhao Sun
α-Chaconine Affects the Apoptosis, Mechanical Barrier Function, and Antioxidant Ability of Mouse Small Intestinal Epithelial Cells
Frontiers in Plant Science
α-chaconine
intestinal epithelial cell
cell cycle
cell apoptosis
mechanical barrier function
antioxidant ability
author_facet Yuhua He
Yuhua He
Yuhua He
Jiaqi Chen
Qiyue Zhang
Jialong Zhang
Lulai Wang
Xiaoxia Chen
Adrian J. Molenaar
Xuezhao Sun
Xuezhao Sun
Xuezhao Sun
author_sort Yuhua He
title α-Chaconine Affects the Apoptosis, Mechanical Barrier Function, and Antioxidant Ability of Mouse Small Intestinal Epithelial Cells
title_short α-Chaconine Affects the Apoptosis, Mechanical Barrier Function, and Antioxidant Ability of Mouse Small Intestinal Epithelial Cells
title_full α-Chaconine Affects the Apoptosis, Mechanical Barrier Function, and Antioxidant Ability of Mouse Small Intestinal Epithelial Cells
title_fullStr α-Chaconine Affects the Apoptosis, Mechanical Barrier Function, and Antioxidant Ability of Mouse Small Intestinal Epithelial Cells
title_full_unstemmed α-Chaconine Affects the Apoptosis, Mechanical Barrier Function, and Antioxidant Ability of Mouse Small Intestinal Epithelial Cells
title_sort α-chaconine affects the apoptosis, mechanical barrier function, and antioxidant ability of mouse small intestinal epithelial cells
publisher Frontiers Media S.A.
series Frontiers in Plant Science
issn 1664-462X
publishDate 2021-06-01
description α-Chaconine is the most abundant glycoalkaloid in potato and toxic to the animal digestive system, but the mechanisms underlying the toxicity are unclear. In this study, mouse small intestinal epithelial cells were incubated with α-chaconine at 0, 0.4, and 0.8 μg/mL for 24, 48, and 72 h to examine apoptosis, mechanical barrier function, and antioxidant ability of the cells using a cell metabolic activity assay, flow cytometry, Western blot, immunofluorescence, and fluorescence quantitative PCR. The results showed that α-chaconine significantly decreased cell proliferation rate, increased apoptosis rate, decreased transepithelial electrical resistance (TEER) value, and increased alkaline phosphatase (AKP) and lactate dehydrogenase (LDH) activities, and there were interactions between α-chaconine concentration and incubation time. α-Chaconine significantly reduced the relative and mRNA expressions of genes coding tight junction proteins zonula occludens-1 (ZO-1) and occludin, increased malondialdehyde (MDA) content, decreased total glutathione (T-GSH) content, reduced the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and γ-glutamylcysteine synthetase (γ-GCS) and the mRNA expressions of SOD, CAT, GSH-Px, and γ-GCS genes. In conclusion, α-chaconine disrupts the cell cycle, destroys the mechanical barrier and permeability of mucosal epithelium, inhibits cell proliferation, and accelerates cell apoptosis.
topic α-chaconine
intestinal epithelial cell
cell cycle
cell apoptosis
mechanical barrier function
antioxidant ability
url https://www.frontiersin.org/articles/10.3389/fpls.2021.673774/full
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