Generation of pigs with a Belgian Blue mutation in MSTN using CRISPR/Cpf1-assisted ssODN-mediated homologous recombination

Generation of pigs with a Belgian Blue mutation in MSTN using CRISPR/Cpf1-assisted ssODN-mediated homologous recombination

CRISPR/Cpf1 has emerged recently as an effective tool for genome editing in many organisms, but its use in pigs to generate precise genetic modifications has seldom been described. Myostatin (MSTN) is a well-characterized negative regulator of muscle development, and natural mutations in this gene c...

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Bibliographic Details
Main Authors: Yun-long ZOU, Zhi-yuan LI, Yun-jing ZOU, Hai-yang HAO, Jia-xiang HU, Ning LI, Qiu-yan LI
Format: Article
Language:English
Published: Elsevier 2019-06-01
Series:Journal of Integrative Agriculture
Subjects:
MSTN
CRISPR/Cpf1
Belgian Blue mutation
genetically modified pigs
single stranded oligodeoxynucleotide
Online Access:http://www.sciencedirect.com/science/article/pii/S2095311919626948

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http://www.sciencedirect.com/science/article/pii/S2095311919626948

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