Neuroprotective Effects of Diets Containing Olive Oil and DHA/EPA in a Mouse Model of Cerebral Ischemia
Stroke is one of the leading causes of death worldwide and while there is increasing evidence that a Mediterranean diet might decrease the risk of a stroke, the effects of dietary fat composition on stroke outcomes have not been fully explored. We hypothesize that the brain damage provoked by a stro...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-05-01
|
Series: | Nutrients |
Subjects: | |
Online Access: | https://www.mdpi.com/2072-6643/11/5/1109 |
id |
doaj-7e6d3dd11b754b179ba61de44c8b74f2 |
---|---|
record_format |
Article |
spelling |
doaj-7e6d3dd11b754b179ba61de44c8b74f22020-11-24T20:46:44ZengMDPI AGNutrients2072-66432019-05-01115110910.3390/nu11051109nu11051109Neuroprotective Effects of Diets Containing Olive Oil and DHA/EPA in a Mouse Model of Cerebral IschemiaRafael Gonzalo-Gobernado0María Irene Ayuso1Loredana Sansone2Juan José Bernal-Jiménez3Víctor Darío Ramos-Herrero4Enrique Sánchez-García5Teresa L. Ramos6Rocío Abia7Francisco J. G. Muriana8Beatriz Bermúdez9Joan Montaner10Neurovascular Research Group, Institute of Biomedicine of Seville, IBiS, Hospital Universitario Virgen del Rocío, Av. Manuel Siurot s/n, 41013 Seville; SpainNeurovascular Research Group, Institute of Biomedicine of Seville, IBiS, Hospital Universitario Virgen del Rocío, Av. Manuel Siurot s/n, 41013 Seville; SpainNeurovascular Research Group, Institute of Biomedicine of Seville, IBiS, Hospital Universitario Virgen del Rocío, Av. Manuel Siurot s/n, 41013 Seville; SpainNeurovascular Research Group, Institute of Biomedicine of Seville, IBiS, Hospital Universitario Virgen del Rocío, Av. Manuel Siurot s/n, 41013 Seville; SpainNeurovascular Research Group, Institute of Biomedicine of Seville, IBiS, Hospital Universitario Virgen del Rocío, Av. Manuel Siurot s/n, 41013 Seville; SpainNeurovascular Research Group, Institute of Biomedicine of Seville, IBiS, Hospital Universitario Virgen del Rocío, Av. Manuel Siurot s/n, 41013 Seville; SpainLaboratory of Cell Therapy and New Therapeutic Targets in Onco-Hematology, Institute of Biomedicine of Seville, IBiS, Hospital Universitario Virgen del Rocío, Av. Manuel Siurot s/n, 41013 Seville, SpainLaboratory of Cellular and Molecular Nutrition, Instituto de la Grasa, CSIC, Ctra. de Utrera Km. 1, 41013 Seville, SpainLaboratory of Cellular and Molecular Nutrition, Instituto de la Grasa, CSIC, Ctra. de Utrera Km. 1, 41013 Seville, SpainDepartment of Cellular Biology, School of Biology, University of Seville, Av. de la Reina Mercedes 6, 41012 Seville, SpainNeurovascular Research Group, Institute of Biomedicine of Seville, IBiS, Hospital Universitario Virgen del Rocío, Av. Manuel Siurot s/n, 41013 Seville; SpainStroke is one of the leading causes of death worldwide and while there is increasing evidence that a Mediterranean diet might decrease the risk of a stroke, the effects of dietary fat composition on stroke outcomes have not been fully explored. We hypothesize that the brain damage provoked by a stroke would be different depending on the source of dietary fat. To test this, male C57BL/6J mice were fed for 4 weeks with a standard low-fat diet (LFD), a high-fat diet (HFD) rich in saturated fatty acids (HFD-SFA), an HFD containing monounsaturated fatty acids (MUFAs) from olive oil (HFD-OO), or an HFD containing MUFAs from olive oil plus polyunsaturated fatty acids (PUFAs) docosahexaenoic acid/eicosapentaenoic acid (DHA/EPA) (HFD-OO-ω3). These mice were then subjected to transient middle cerebral artery occlusion (tMCAo). Behavioural tests and histological analyses were performed 24 and/or 48 h after tMCAo in order to elucidate the impact of these diets with different fatty acid profiles on the ischemic lesion and on neurological functions. Mice fed with HFD-OO-ω3 displayed better histological outcomes after cerebral ischemia than mice that received an HFD-SFA or LFD. Furthermore, PUFA- and MUFA-enriched diets improved the motor function and neurological performance of ischemic mice relative to those fed with an LFD or HFD-SFA. These findings support the use of DHA/EPA-omega-3-fatty acid supplementation and olive oil as dietary source of MUFAs in order to reduce the damage and protect the brain when a stroke occurs.https://www.mdpi.com/2072-6643/11/5/1109cerebral ischemiastrokemiddle cerebral artery occlusion (MCAo)omega-3 fatty acidsDHAEPAolive oilMUFAPUFASFAneuroprotectionneurological functionbehaviour |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rafael Gonzalo-Gobernado María Irene Ayuso Loredana Sansone Juan José Bernal-Jiménez Víctor Darío Ramos-Herrero Enrique Sánchez-García Teresa L. Ramos Rocío Abia Francisco J. G. Muriana Beatriz Bermúdez Joan Montaner |
spellingShingle |
Rafael Gonzalo-Gobernado María Irene Ayuso Loredana Sansone Juan José Bernal-Jiménez Víctor Darío Ramos-Herrero Enrique Sánchez-García Teresa L. Ramos Rocío Abia Francisco J. G. Muriana Beatriz Bermúdez Joan Montaner Neuroprotective Effects of Diets Containing Olive Oil and DHA/EPA in a Mouse Model of Cerebral Ischemia Nutrients cerebral ischemia stroke middle cerebral artery occlusion (MCAo) omega-3 fatty acids DHA EPA olive oil MUFA PUFA SFA neuroprotection neurological function behaviour |
author_facet |
Rafael Gonzalo-Gobernado María Irene Ayuso Loredana Sansone Juan José Bernal-Jiménez Víctor Darío Ramos-Herrero Enrique Sánchez-García Teresa L. Ramos Rocío Abia Francisco J. G. Muriana Beatriz Bermúdez Joan Montaner |
author_sort |
Rafael Gonzalo-Gobernado |
title |
Neuroprotective Effects of Diets Containing Olive Oil and DHA/EPA in a Mouse Model of Cerebral Ischemia |
title_short |
Neuroprotective Effects of Diets Containing Olive Oil and DHA/EPA in a Mouse Model of Cerebral Ischemia |
title_full |
Neuroprotective Effects of Diets Containing Olive Oil and DHA/EPA in a Mouse Model of Cerebral Ischemia |
title_fullStr |
Neuroprotective Effects of Diets Containing Olive Oil and DHA/EPA in a Mouse Model of Cerebral Ischemia |
title_full_unstemmed |
Neuroprotective Effects of Diets Containing Olive Oil and DHA/EPA in a Mouse Model of Cerebral Ischemia |
title_sort |
neuroprotective effects of diets containing olive oil and dha/epa in a mouse model of cerebral ischemia |
publisher |
MDPI AG |
series |
Nutrients |
issn |
2072-6643 |
publishDate |
2019-05-01 |
description |
Stroke is one of the leading causes of death worldwide and while there is increasing evidence that a Mediterranean diet might decrease the risk of a stroke, the effects of dietary fat composition on stroke outcomes have not been fully explored. We hypothesize that the brain damage provoked by a stroke would be different depending on the source of dietary fat. To test this, male C57BL/6J mice were fed for 4 weeks with a standard low-fat diet (LFD), a high-fat diet (HFD) rich in saturated fatty acids (HFD-SFA), an HFD containing monounsaturated fatty acids (MUFAs) from olive oil (HFD-OO), or an HFD containing MUFAs from olive oil plus polyunsaturated fatty acids (PUFAs) docosahexaenoic acid/eicosapentaenoic acid (DHA/EPA) (HFD-OO-ω3). These mice were then subjected to transient middle cerebral artery occlusion (tMCAo). Behavioural tests and histological analyses were performed 24 and/or 48 h after tMCAo in order to elucidate the impact of these diets with different fatty acid profiles on the ischemic lesion and on neurological functions. Mice fed with HFD-OO-ω3 displayed better histological outcomes after cerebral ischemia than mice that received an HFD-SFA or LFD. Furthermore, PUFA- and MUFA-enriched diets improved the motor function and neurological performance of ischemic mice relative to those fed with an LFD or HFD-SFA. These findings support the use of DHA/EPA-omega-3-fatty acid supplementation and olive oil as dietary source of MUFAs in order to reduce the damage and protect the brain when a stroke occurs. |
topic |
cerebral ischemia stroke middle cerebral artery occlusion (MCAo) omega-3 fatty acids DHA EPA olive oil MUFA PUFA SFA neuroprotection neurological function behaviour |
url |
https://www.mdpi.com/2072-6643/11/5/1109 |
work_keys_str_mv |
AT rafaelgonzalogobernado neuroprotectiveeffectsofdietscontainingoliveoilanddhaepainamousemodelofcerebralischemia AT mariaireneayuso neuroprotectiveeffectsofdietscontainingoliveoilanddhaepainamousemodelofcerebralischemia AT loredanasansone neuroprotectiveeffectsofdietscontainingoliveoilanddhaepainamousemodelofcerebralischemia AT juanjosebernaljimenez neuroprotectiveeffectsofdietscontainingoliveoilanddhaepainamousemodelofcerebralischemia AT victordarioramosherrero neuroprotectiveeffectsofdietscontainingoliveoilanddhaepainamousemodelofcerebralischemia AT enriquesanchezgarcia neuroprotectiveeffectsofdietscontainingoliveoilanddhaepainamousemodelofcerebralischemia AT teresalramos neuroprotectiveeffectsofdietscontainingoliveoilanddhaepainamousemodelofcerebralischemia AT rocioabia neuroprotectiveeffectsofdietscontainingoliveoilanddhaepainamousemodelofcerebralischemia AT franciscojgmuriana neuroprotectiveeffectsofdietscontainingoliveoilanddhaepainamousemodelofcerebralischemia AT beatrizbermudez neuroprotectiveeffectsofdietscontainingoliveoilanddhaepainamousemodelofcerebralischemia AT joanmontaner neuroprotectiveeffectsofdietscontainingoliveoilanddhaepainamousemodelofcerebralischemia |
_version_ |
1716811710103289856 |