Evaluation of Reference Genes and Normalization Strategy for Quantitative Real-Time PCR in Human Pancreatic Carcinoma

Histologically verified pairs (n = 10) of pancreatic tumors and non-neoplastic tissues were used for quantitative real-time PCR and the stability of 24 reference genes was analyzed with geNorm and NormFinder software. Raw Cq values correlated with the degree of RNA degradation. This correlation was...

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Bibliographic Details
Main Authors: Beatrice Mohelnikova-Duchonova, Martin Oliverius, Eva Honsova, Pavel Soucek
Format: Article
Language:English
Published: Hindawi Limited 2012-01-01
Series:Disease Markers
Online Access:http://dx.doi.org/10.3233/DMA-2011-0875