A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system

A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system

CRISPR/Cas9 can be used as an experimental tool to inactivate genes in cells. However, a CRISPR-targeted cell population will not show a uniform genotype of the targeted gene. Instead, a mix of genotypes is generated - from wild type to different forms of insertions and deletions. Such mixed genotyp...

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Bibliographic Details
Main Authors: Yunbing Shen, Long Jiang, Vaishnavi Srinivasan Iyer, Bruno Raposo, Anatoly Dubnovitsky, Sanjaykumar V. Boddul, Zsolt Kasza, Fredrik Wermeling
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:Computational and Structural Biotechnology Journal
Subjects:
CRISPR
Sequence analysis
Drug target discovery
Cell assay
In vivo model
Hematopoiesis
Online Access:http://www.sciencedirect.com/science/article/pii/S2001037021004050

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http://www.sciencedirect.com/science/article/pii/S2001037021004050

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