Evaluation of RNA extraction methods in rice and their application in expression analysis of resistance genes against Magnaporthe oryzae

Evaluation of RNA extraction methods in rice and their application in expression analysis of resistance genes against Magnaporthe oryzae

Extraction of RNA of high quality and integrity is essential for gene expression studies and all downstream RNA-based techniques. The leaves of 16 merit Malaysian rice varieties were used to isolate total RNA using five different methods. The quantity, quality and integrity of extracted RNA were con...

Full description

Bibliographic Details
Main Authors: Parisa Azizi, Mohd Y. Rafii, Maziah Mahmood, Siti Nor Akmar Abdullah, Mohamed Musa Hanafi, Muhammad Abdul Latif, Mahbod Sahebi, Sadegh Ashkani
Format: Article
Language:English
Published: Taylor & Francis Group 2017-01-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
Resistance genes
Magnaporthe oryzae
real-time PCR
semi-quantitative PCR
Online Access:http://dx.doi.org/10.1080/13102818.2016.1259015

Internet

http://dx.doi.org/10.1080/13102818.2016.1259015

Similar Items