‘Cold shock’ increases the frequency of homology directed repair gene editing in induced pluripotent stem cells

‘Cold shock’ increases the frequency of homology directed repair gene editing in induced pluripotent stem cells

Abstract Using CRISPR/Cas9 delivered as a RNA modality in conjunction with a lipid specifically formulated for large RNA molecules, we demonstrate that homology directed repair (HDR) rates between 20–40% can be achieved in induced pluripotent stem cells (iPSC). Furthermore, low HDR rates (between 1–...

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Bibliographic Details
Main Authors: Q. Guo, G. Mintier, M. Ma-Edmonds, D. Storton, X. Wang, X. Xiao, B Kienzle, D. Zhao, John N. Feder
Format: Article
Language:English
Published: Nature Publishing Group 2018-02-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-018-20358-5

Internet

https://doi.org/10.1038/s41598-018-20358-5

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