Improving stool sample processing and pyrosequencing for quantifying benzimidazole resistance alleles in Trichuris trichiura and Necator americanus pooled eggs

Improving stool sample processing and pyrosequencing for quantifying benzimidazole resistance alleles in Trichuris trichiura and Necator americanus pooled eggs

Abstract Background There is an urgent need for an extensive evaluation of benzimidazole efficacy in humans. In veterinary science, benzimidazole resistance has been mainly associated with three single-nucleotide polymorphisms (SNPs) in the isotype-1 β-tubulin gene. In this study, we optimized the s...

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Bibliographic Details
Main Authors: Javier Gandasegui, Berta Grau-Pujol, María Cambra-Pelleja, Valdemiro Escola, Maria Antonietta Demontis, Anelsio Cossa, José Carlos Jamine, Rafael Balaña-Fouce, Lisette van Lieshout, José Muñoz, María Martínez-Valladares, The Stopping Transmission of Intestinal Parasites (STOP) Project Consortium
Format: Article
Language:English
Published: BMC 2021-09-01
Series:Parasites & Vectors
Subjects:
Soil-transmitted helminths
Benzimidazoles
Anthelmintic resistance
Pyrosequencing
Online Access:https://doi.org/10.1186/s13071-021-04941-w

Internet

https://doi.org/10.1186/s13071-021-04941-w

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