Improving stool sample processing and pyrosequencing for quantifying benzimidazole resistance alleles in Trichuris trichiura and Necator americanus pooled eggs
Abstract Background There is an urgent need for an extensive evaluation of benzimidazole efficacy in humans. In veterinary science, benzimidazole resistance has been mainly associated with three single-nucleotide polymorphisms (SNPs) in the isotype-1 β-tubulin gene. In this study, we optimized the s...
Main Authors: | Javier Gandasegui, Berta Grau-Pujol, María Cambra-Pelleja, Valdemiro Escola, Maria Antonietta Demontis, Anelsio Cossa, José Carlos Jamine, Rafael Balaña-Fouce, Lisette van Lieshout, José Muñoz, María Martínez-Valladares, The Stopping Transmission of Intestinal Parasites (STOP) Project Consortium |
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Format: | Article |
Language: | English |
Published: |
BMC
2021-09-01
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Series: | Parasites & Vectors |
Subjects: | |
Online Access: | https://doi.org/10.1186/s13071-021-04941-w |
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