The SMC Loader Scc2 Promotes ncRNA Biogenesis and Translational Fidelity.
The Scc2-Scc4 complex is essential for loading the cohesin complex onto DNA. Cohesin has important roles in chromosome segregation, DSB repair, and chromosome condensation. Here we report that Scc2 is important for gene expression in budding yeast. Scc2 and the transcriptional regulator Paf1 collabo...
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doaj-7f45a7651deb4b679623900e031be4ff2020-11-24T21:41:59ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042015-07-01117e100530810.1371/journal.pgen.1005308The SMC Loader Scc2 Promotes ncRNA Biogenesis and Translational Fidelity.Musinu ZakariRhonda Trimble RossAllison PeakMarco BlanchetteChris SeidelJennifer L GertonThe Scc2-Scc4 complex is essential for loading the cohesin complex onto DNA. Cohesin has important roles in chromosome segregation, DSB repair, and chromosome condensation. Here we report that Scc2 is important for gene expression in budding yeast. Scc2 and the transcriptional regulator Paf1 collaborate to promote the production of Box H/ACA snoRNAs which guide pseudouridylation of RNAs including ribosomal RNA. Mutation of SCC2 was associated with defects in the production of ribosomal RNA, ribosome assembly, and splicing. While the scc2 mutant does not have a general defect in protein synthesis, it shows increased frameshifting and reduced cap-independent translation. These findings suggest Scc2 normally promotes a gene expression program that supports translational fidelity. We hypothesize that translational dysfunction may contribute to the human disorder Cornelia de Lange syndrome, which is caused by mutations in NIPBL, the human ortholog of SCC2.http://europepmc.org/articles/PMC4503661?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Musinu Zakari Rhonda Trimble Ross Allison Peak Marco Blanchette Chris Seidel Jennifer L Gerton |
spellingShingle |
Musinu Zakari Rhonda Trimble Ross Allison Peak Marco Blanchette Chris Seidel Jennifer L Gerton The SMC Loader Scc2 Promotes ncRNA Biogenesis and Translational Fidelity. PLoS Genetics |
author_facet |
Musinu Zakari Rhonda Trimble Ross Allison Peak Marco Blanchette Chris Seidel Jennifer L Gerton |
author_sort |
Musinu Zakari |
title |
The SMC Loader Scc2 Promotes ncRNA Biogenesis and Translational Fidelity. |
title_short |
The SMC Loader Scc2 Promotes ncRNA Biogenesis and Translational Fidelity. |
title_full |
The SMC Loader Scc2 Promotes ncRNA Biogenesis and Translational Fidelity. |
title_fullStr |
The SMC Loader Scc2 Promotes ncRNA Biogenesis and Translational Fidelity. |
title_full_unstemmed |
The SMC Loader Scc2 Promotes ncRNA Biogenesis and Translational Fidelity. |
title_sort |
smc loader scc2 promotes ncrna biogenesis and translational fidelity. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Genetics |
issn |
1553-7390 1553-7404 |
publishDate |
2015-07-01 |
description |
The Scc2-Scc4 complex is essential for loading the cohesin complex onto DNA. Cohesin has important roles in chromosome segregation, DSB repair, and chromosome condensation. Here we report that Scc2 is important for gene expression in budding yeast. Scc2 and the transcriptional regulator Paf1 collaborate to promote the production of Box H/ACA snoRNAs which guide pseudouridylation of RNAs including ribosomal RNA. Mutation of SCC2 was associated with defects in the production of ribosomal RNA, ribosome assembly, and splicing. While the scc2 mutant does not have a general defect in protein synthesis, it shows increased frameshifting and reduced cap-independent translation. These findings suggest Scc2 normally promotes a gene expression program that supports translational fidelity. We hypothesize that translational dysfunction may contribute to the human disorder Cornelia de Lange syndrome, which is caused by mutations in NIPBL, the human ortholog of SCC2. |
url |
http://europepmc.org/articles/PMC4503661?pdf=render |
work_keys_str_mv |
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