Improving the thermostability of a fungal GH11 xylanase via site-directed mutagenesis guided by sequence and structural analysis

Improving the thermostability of a fungal GH11 xylanase via site-directed mutagenesis guided by sequence and structural analysis

Abstract Background Xylanases have been widely employed in many industrial processes, and thermophilic xylanases are in great demand for meeting the high-temperature requirements of biotechnological treatments. In this work, we aim to improve the thermostability of XynCDBFV, a glycoside hydrolase (G...

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Bibliographic Details
Main Authors: Nanyu Han, Huabiao Miao, Junmei Ding, Junjun Li, Yuelin Mu, Junpei Zhou, Zunxi Huang
Format: Article
Language:English
Published: BMC 2017-05-01
Series:Biotechnology for Biofuels
Subjects:
Xylanase
Thermostability
B-factor
MD simulation
Site-directed mutagenesis
C-terminus replacement
Online Access:http://link.springer.com/article/10.1186/s13068-017-0824-y

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http://link.springer.com/article/10.1186/s13068-017-0824-y

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