SPLICEFINDER - a fast and easy screening method for active protein trans-splicing positions.

Split intein enabled protein trans-splicing (PTS) is a powerful method for the ligation of two protein fragments, thereby paving the way for various protein modification or protein function control applications. PTS activity is strongly influenced by the amino acids directly flanking the splice junc...

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Bibliographic Details
Main Authors: Joachim Zettler, Simone Eppmann, Alena Busche, Dina Dikovskaya, Volker Dötsch, Henning D Mootz, Tim Sonntag
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3759424?pdf=render