Profiling of Phenolic Compounds and Antioxidant Activity of 12 Cruciferous Vegetables
The phenolic profiles of 12 cruciferous vegetables (pakchoi, choysum, Chinese cabbage, kailan, Brussels sprout, cabbage, cauliflower, broccoli, rocket salad, red cherry radish, daikon radish, and watercress) were studied with UHPLC-MS/MS. Antioxidant activity and total phenolic content (TPC) were al...
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doaj-7ff4a1ae400649509bdc1fa7d511f3fd2020-11-24T21:42:55ZengMDPI AGMolecules1420-30492018-05-01235113910.3390/molecules23051139molecules23051139Profiling of Phenolic Compounds and Antioxidant Activity of 12 Cruciferous VegetablesZhifeng Li0Hui Wen Lee1Xu Liang2Dong Liang3Qi Wang4Dejian Huang5Choon Nam Ong6National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, ChinaNUS Environmental Research Institute, National University of Singapore, 5A Engineering Drive 1, Singapore 117411, SingaporeNUS Environmental Research Institute, National University of Singapore, 5A Engineering Drive 1, Singapore 117411, SingaporeSaw Swee Hock School of Public Health, National University of Singapore, 12 Science Drive 2, Singapore 117549, SingaporeNational Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, No. 818 Yunwan Road, Nanchang 330002, ChinaFood Science and Technology Program, Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, SingaporeNUS Environmental Research Institute, National University of Singapore, 5A Engineering Drive 1, Singapore 117411, SingaporeThe phenolic profiles of 12 cruciferous vegetables (pakchoi, choysum, Chinese cabbage, kailan, Brussels sprout, cabbage, cauliflower, broccoli, rocket salad, red cherry radish, daikon radish, and watercress) were studied with UHPLC-MS/MS. Antioxidant activity and total phenolic content (TPC) were also evaluated. A total of 74 phenolic compounds were identified, including 16 hydroxycinnamic acids and derivatives, and 58 flavonoids and derivatives. The main flavonoids identified were glycosylated quercetin, kaempferol and isorhamnetin, and the main hydroxycinnamic acids were ferulic, sinapic, caffeic and p-coumaric acids. Principal component analysis (PCA) revealed that the distribution of phenolic compounds in different genera of cruciferous vegetables was in accordance with their conventional taxonomy. The DPPH, ORAC and TPC values ranged from 1.11 to 9.54 µmoles Trolox equivalent/g FW, 5.34 to 32.92 µmoles Trolox equivalent/g FW, and 0.16 to 1.93 mg gallic acid equivalent/g FW respectively. Spearman’s correlation showed significant (p < 0.05) positive correlations between TPC, flavonoids and antioxidant activity.http://www.mdpi.com/1420-3049/23/5/1139Brassicaceae vegetablescruciferous vegetablesphenolic compoundsantioxidant activityUHPLC-MS/MSprincipal component analysis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhifeng Li Hui Wen Lee Xu Liang Dong Liang Qi Wang Dejian Huang Choon Nam Ong |
spellingShingle |
Zhifeng Li Hui Wen Lee Xu Liang Dong Liang Qi Wang Dejian Huang Choon Nam Ong Profiling of Phenolic Compounds and Antioxidant Activity of 12 Cruciferous Vegetables Molecules Brassicaceae vegetables cruciferous vegetables phenolic compounds antioxidant activity UHPLC-MS/MS principal component analysis |
author_facet |
Zhifeng Li Hui Wen Lee Xu Liang Dong Liang Qi Wang Dejian Huang Choon Nam Ong |
author_sort |
Zhifeng Li |
title |
Profiling of Phenolic Compounds and Antioxidant Activity of 12 Cruciferous Vegetables |
title_short |
Profiling of Phenolic Compounds and Antioxidant Activity of 12 Cruciferous Vegetables |
title_full |
Profiling of Phenolic Compounds and Antioxidant Activity of 12 Cruciferous Vegetables |
title_fullStr |
Profiling of Phenolic Compounds and Antioxidant Activity of 12 Cruciferous Vegetables |
title_full_unstemmed |
Profiling of Phenolic Compounds and Antioxidant Activity of 12 Cruciferous Vegetables |
title_sort |
profiling of phenolic compounds and antioxidant activity of 12 cruciferous vegetables |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2018-05-01 |
description |
The phenolic profiles of 12 cruciferous vegetables (pakchoi, choysum, Chinese cabbage, kailan, Brussels sprout, cabbage, cauliflower, broccoli, rocket salad, red cherry radish, daikon radish, and watercress) were studied with UHPLC-MS/MS. Antioxidant activity and total phenolic content (TPC) were also evaluated. A total of 74 phenolic compounds were identified, including 16 hydroxycinnamic acids and derivatives, and 58 flavonoids and derivatives. The main flavonoids identified were glycosylated quercetin, kaempferol and isorhamnetin, and the main hydroxycinnamic acids were ferulic, sinapic, caffeic and p-coumaric acids. Principal component analysis (PCA) revealed that the distribution of phenolic compounds in different genera of cruciferous vegetables was in accordance with their conventional taxonomy. The DPPH, ORAC and TPC values ranged from 1.11 to 9.54 µmoles Trolox equivalent/g FW, 5.34 to 32.92 µmoles Trolox equivalent/g FW, and 0.16 to 1.93 mg gallic acid equivalent/g FW respectively. Spearman’s correlation showed significant (p < 0.05) positive correlations between TPC, flavonoids and antioxidant activity. |
topic |
Brassicaceae vegetables cruciferous vegetables phenolic compounds antioxidant activity UHPLC-MS/MS principal component analysis |
url |
http://www.mdpi.com/1420-3049/23/5/1139 |
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