Stabilization of cyclohexanone monooxygenase by a computationally designed disulfide bond spanning only one residue

Stabilization of cyclohexanone monooxygenase by a computationally designed disulfide bond spanning only one residue

Enzyme stability is an important parameter in biocatalytic applications, and there is a strong need for efficient methods to generate robust enzymes. We investigated whether stabilizing disulfide bonds can be computationally designed based on a model structure. In our approach, unlike in previous di...

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Bibliographic Details
Main Authors: Hugo L. van Beek, Hein J. Wijma, Lucie Fromont, Dick B. Janssen, Marco W. Fraaije
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:FEBS Open Bio
Subjects:
Baeyer–Villiger monooxygenase
Computational design
Disulfide bonds
Thermostability
Flavoprotein
Enzyme engineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2211546314000102

Internet

http://www.sciencedirect.com/science/article/pii/S2211546314000102

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