Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle

The engineering of methanol-dependent growth in Escherichia coli is challenging. Here, the authors predict and experimentally validate methanol-dependent strains with a complete RuMP cycle and high potential for the development of a methylotrophic platform organism.

Bibliographic Details
Main Authors: Philipp Keller, Elad Noor, Fabian Meyer, Michael A. Reiter, Stanislav Anastassov, Patrick Kiefer, Julia A. Vorholt
Format: Article
Language:English
Published: Nature Publishing Group 2020-10-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-19235-5