Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle

The engineering of methanol-dependent growth in Escherichia coli is challenging. Here, the authors predict and experimentally validate methanol-dependent strains with a complete RuMP cycle and high potential for the development of a methylotrophic platform organism.

Bibliographic Details
Main Authors:	Philipp Keller, Elad Noor, Fabian Meyer, Michael A. Reiter, Stanislav Anastassov, Patrick Kiefer, Julia A. Vorholt
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-10-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-19235-5

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spelling	doaj-8021e3394aee40be935ad830142840df2021-05-11T08:58:10ZengNature Publishing GroupNature Communications2041-17232020-10-0111111010.1038/s41467-020-19235-5Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cyclePhilipp Keller0Elad Noor1Fabian Meyer2Michael A. Reiter3Stanislav Anastassov4Patrick Kiefer5Julia A. Vorholt6Institute of Microbiology, Department of Biology, ETH ZurichInstitute of Molecular Systems Biology, Department of Biology, ETH ZurichInstitute of Microbiology, Department of Biology, ETH ZurichInstitute of Microbiology, Department of Biology, ETH ZurichInstitute of Microbiology, Department of Biology, ETH ZurichInstitute of Microbiology, Department of Biology, ETH ZurichInstitute of Microbiology, Department of Biology, ETH ZurichThe engineering of methanol-dependent growth in Escherichia coli is challenging. Here, the authors predict and experimentally validate methanol-dependent strains with a complete RuMP cycle and high potential for the development of a methylotrophic platform organism.https://doi.org/10.1038/s41467-020-19235-5
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Philipp Keller Elad Noor Fabian Meyer Michael A. Reiter Stanislav Anastassov Patrick Kiefer Julia A. Vorholt
spellingShingle	Philipp Keller Elad Noor Fabian Meyer Michael A. Reiter Stanislav Anastassov Patrick Kiefer Julia A. Vorholt Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle Nature Communications
author_facet	Philipp Keller Elad Noor Fabian Meyer Michael A. Reiter Stanislav Anastassov Patrick Kiefer Julia A. Vorholt
author_sort	Philipp Keller
title	Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle
title_short	Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle
title_full	Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle
title_fullStr	Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle
title_full_unstemmed	Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle
title_sort	methanol-dependent escherichia coli strains with a complete ribulose monophosphate cycle
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-10-01
description	The engineering of methanol-dependent growth in Escherichia coli is challenging. Here, the authors predict and experimentally validate methanol-dependent strains with a complete RuMP cycle and high potential for the development of a methylotrophic platform organism.
url	https://doi.org/10.1038/s41467-020-19235-5
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Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle

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