A method for the immortalization of newborn mouse skin keratinocytes

Isolation and culture of mouse primary epidermal keratinocytes is a common technique that allows for easy genetic and environmental manipulation. However, due to their limited lifespan in culture, experiments utilizing primary keratinocytes require large numbers of animals, and are time consuming an...

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Bibliographic Details
Main Authors: Brianna O Hammiller, Taghrid Bahig El-Abaseri, Andrzej A Dlugosz, Laura A Hansen
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-07-01
Series:Frontiers in Oncology
Subjects:
Online Access:http://journal.frontiersin.org/Journal/10.3389/fonc.2015.00177/full