Improving the Accuracy of Flow Cytometric Quantification of Microbial Populations in Sediments: Importance of Cell Staining Procedures

Improving the Accuracy of Flow Cytometric Quantification of Microbial Populations in Sediments: Importance of Cell Staining Procedures

The accuracy of flow cytometric (FCM) quantifications of microbial populations in sediments varies with FCM settings, cell extraction and staining protocols, as well as sample types. In the present study, we improve the accuracy of FCM for enumerating microorganisms inhabiting diverse lake and marin...

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Bibliographic Details
Main Authors: Longhui Deng, Annika Fiskal, Xingguo Han, Nathalie Dubois, Stefano Michele Bernasconi, Mark Alexander Lever
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-04-01
Series:Frontiers in Microbiology
Subjects:
microbial populations
lacustrine
marine
cell counts
staining technique
flow cytometry
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.00720/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.00720/full

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