A multiplexed nanostructure-initiator mass spectrometry (NIMS) assay for simultaneously detecting glycosyl hydrolase and lignin modifying enzyme activities

Abstract Lignocellulosic biomass is composed of three major biopolymers: cellulose, hemicellulose and lignin. Analytical tools capable of quickly detecting both glycan and lignin deconstruction are needed to support the development and characterization of efficient enzymes/enzyme cocktails. Previous...

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Bibliographic Details
Main Authors: Nicole Ing, Kai Deng, Yan Chen, Martina Aulitto, Jennifer W. Gin, Thanh Le Mai Pham, Christopher J. Petzold, Steve W. Singer, Benjamin Bowen, Kenneth L. Sale, Blake A. Simmons, Anup K. Singh, Paul D. Adams, Trent R. Northen
Format: Article
Language:English
Published: Nature Publishing Group 2021-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-91181-8