In vitro bactericidal, antidiabetic, cytotoxic, anticoagulant, and hemolytic effect of green-synthesized silver nanoparticles using Allium sativum clove extract incubated at various temperatures
The current research aimed to evaluate in vitro biological activities of green-synthesized silver nanoparticles using the Allium sativum clove extract. The stability of green-synthesized silver nanoparticles was evaluated via storage at 4°C, room temperature (37°C), and calcined at 300°C, 500°C, and...
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2020-10-01
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Online Access: | https://doi.org/10.1515/gps-2020-0051 |
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doaj-80cd0d68ec814da7a50b7dc196ea46222021-10-02T17:47:23ZengDe GruyterGreen Processing and Synthesis2191-95422191-95502020-10-019153855310.1515/gps-2020-0051gps-2020-0051In vitro bactericidal, antidiabetic, cytotoxic, anticoagulant, and hemolytic effect of green-synthesized silver nanoparticles using Allium sativum clove extract incubated at various temperaturesAndleeb Saiqa0Tariq Faiza1Muneer Areesha2Nazir Tooba3Shahid Beenish4Latif Zahid5Abbasi Shahab Ahmed6Haq Ihsan ul7Majeed Zahid8Khan Salah Ud-Din9Khan Shahab Ud-Din10Khan Taj Muhammad11Al Farraj Dunia A.12Department of Zoology, Microbial Biotechnology Laboratory, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, PakistanDepartment of Zoology, Microbial Biotechnology Laboratory, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, PakistanDepartment of Zoology, Microbial Biotechnology Laboratory, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, PakistanDepartment of Zoology, Microbial Biotechnology Laboratory, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, PakistanDepartment of Zoology, Physiology Laboratory, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, PakistanDepartment of Zoology, Microbial Biotechnology Laboratory, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, PakistanDepartment of Physics, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, PakistanDepartment of Pharmaceutical Sciences, Quaid-i-Azam University, Islamabad, PakistanDepartment of Biotechnology, University of Azad Jammu and Kashmir, Muzaffarabad, 13100, PakistanSustainable Energy Technologies (SET) Center, College of Engineering, King Saud University, PO-Box 800, Riyadh, 11421, Saudi ArabiaNational Tokamak Fusion Program, P.O. Nilore, 44000, Islamabad, PakistanNational Institute of Lasers and Optronics (NILOP), P.O. Nilore, 45650, Islamabad, PakistanDepartment of Botany and Microbiology, College of Science, King Saud University, PO-Box 800, Riyadh, 11421, Saudi ArabiaThe current research aimed to evaluate in vitro biological activities of green-synthesized silver nanoparticles using the Allium sativum clove extract. The stability of green-synthesized silver nanoparticles was evaluated via storage at 4°C, room temperature (37°C), and calcined at 300°C, 500°C, and 700°C. The antibacterial effect was evaluated using agar well, spread plate, biofilm reduction, and cell proliferation inhibition assays. The cytotoxic and antidiabetic effects were determined via brine shrimp lethality, protein kinase inhibition, and α-amylase inhibition assays. DPPH scavenging, iron-chelating, anticoagulant, and hemolytic effects were evaluated. The highest inhibition of Klebsiella pneumoniae was observed when freshly prepared, calcined (300°C), and stored nanoparticles (4°C and 37°C) were applied (9.66, 9.55, 7.33, and 6.65 mm) against freshly prepared and calcined at 700°C which showed the highest inhibition of Pseudomonas aeruginosa (8.55 and 7.66 mm). Cell viability assay, biofilm reduction assay, and spread plate method showed a significant antibacterial effect of freshly prepared silver nanoparticles. Freshly prepared and calcined nanoparticles at 300°C and 500°C possessed strong antioxidant and iron-chelating activity. Among all the synthesized silver nanoparticles, freshly prepared and calcined nanoparticles (300°C and 500°C) increases the prothrombin time. Silver nanoparticles possessed significant anticoagulant properties and less toxic at least concentration toward human RBCs. In brine shrimp lethality assay, freshly prepared nanoparticles showed a stronger toxic effect and caused high mortality of larvae. Protein kinase inhibition assay revealed that freshly prepared nanoparticles had the highest zone of inhibition (18.0 mm) at 50 µg/disc. Green-synthesized nanoparticles would be used as potential therapeutic agents to overcome both infectious and noninfectious diseases.https://doi.org/10.1515/gps-2020-0051antibacterial effectantidiabetic effectanticoagulant effectsilver nanoparticlesallium sativum |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Andleeb Saiqa Tariq Faiza Muneer Areesha Nazir Tooba Shahid Beenish Latif Zahid Abbasi Shahab Ahmed Haq Ihsan ul Majeed Zahid Khan Salah Ud-Din Khan Shahab Ud-Din Khan Taj Muhammad Al Farraj Dunia A. |
spellingShingle |
Andleeb Saiqa Tariq Faiza Muneer Areesha Nazir Tooba Shahid Beenish Latif Zahid Abbasi Shahab Ahmed Haq Ihsan ul Majeed Zahid Khan Salah Ud-Din Khan Shahab Ud-Din Khan Taj Muhammad Al Farraj Dunia A. In vitro bactericidal, antidiabetic, cytotoxic, anticoagulant, and hemolytic effect of green-synthesized silver nanoparticles using Allium sativum clove extract incubated at various temperatures Green Processing and Synthesis antibacterial effect antidiabetic effect anticoagulant effect silver nanoparticles allium sativum |
author_facet |
Andleeb Saiqa Tariq Faiza Muneer Areesha Nazir Tooba Shahid Beenish Latif Zahid Abbasi Shahab Ahmed Haq Ihsan ul Majeed Zahid Khan Salah Ud-Din Khan Shahab Ud-Din Khan Taj Muhammad Al Farraj Dunia A. |
author_sort |
Andleeb Saiqa |
title |
In vitro bactericidal, antidiabetic, cytotoxic, anticoagulant, and hemolytic effect of green-synthesized silver nanoparticles using Allium sativum clove extract incubated at various temperatures |
title_short |
In vitro bactericidal, antidiabetic, cytotoxic, anticoagulant, and hemolytic effect of green-synthesized silver nanoparticles using Allium sativum clove extract incubated at various temperatures |
title_full |
In vitro bactericidal, antidiabetic, cytotoxic, anticoagulant, and hemolytic effect of green-synthesized silver nanoparticles using Allium sativum clove extract incubated at various temperatures |
title_fullStr |
In vitro bactericidal, antidiabetic, cytotoxic, anticoagulant, and hemolytic effect of green-synthesized silver nanoparticles using Allium sativum clove extract incubated at various temperatures |
title_full_unstemmed |
In vitro bactericidal, antidiabetic, cytotoxic, anticoagulant, and hemolytic effect of green-synthesized silver nanoparticles using Allium sativum clove extract incubated at various temperatures |
title_sort |
in vitro bactericidal, antidiabetic, cytotoxic, anticoagulant, and hemolytic effect of green-synthesized silver nanoparticles using allium sativum clove extract incubated at various temperatures |
publisher |
De Gruyter |
series |
Green Processing and Synthesis |
issn |
2191-9542 2191-9550 |
publishDate |
2020-10-01 |
description |
The current research aimed to evaluate in vitro biological activities of green-synthesized silver nanoparticles using the Allium sativum clove extract. The stability of green-synthesized silver nanoparticles was evaluated via storage at 4°C, room temperature (37°C), and calcined at 300°C, 500°C, and 700°C. The antibacterial effect was evaluated using agar well, spread plate, biofilm reduction, and cell proliferation inhibition assays. The cytotoxic and antidiabetic effects were determined via brine shrimp lethality, protein kinase inhibition, and α-amylase inhibition assays. DPPH scavenging, iron-chelating, anticoagulant, and hemolytic effects were evaluated. The highest inhibition of Klebsiella pneumoniae was observed when freshly prepared, calcined (300°C), and stored nanoparticles (4°C and 37°C) were applied (9.66, 9.55, 7.33, and 6.65 mm) against freshly prepared and calcined at 700°C which showed the highest inhibition of Pseudomonas aeruginosa (8.55 and 7.66 mm). Cell viability assay, biofilm reduction assay, and spread plate method showed a significant antibacterial effect of freshly prepared silver nanoparticles. Freshly prepared and calcined nanoparticles at 300°C and 500°C possessed strong antioxidant and iron-chelating activity. Among all the synthesized silver nanoparticles, freshly prepared and calcined nanoparticles (300°C and 500°C) increases the prothrombin time. Silver nanoparticles possessed significant anticoagulant properties and less toxic at least concentration toward human RBCs. In brine shrimp lethality assay, freshly prepared nanoparticles showed a stronger toxic effect and caused high mortality of larvae. Protein kinase inhibition assay revealed that freshly prepared nanoparticles had the highest zone of inhibition (18.0 mm) at 50 µg/disc. Green-synthesized nanoparticles would be used as potential therapeutic agents to overcome both infectious and noninfectious diseases. |
topic |
antibacterial effect antidiabetic effect anticoagulant effect silver nanoparticles allium sativum |
url |
https://doi.org/10.1515/gps-2020-0051 |
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